Page 65 - Read Online
P. 65
Page 8 of 11 Narayanan et al. J Cancer Metastasis Treat 2019;5:36 I http://dx.doi.org/10.20517/2394-4722.2018.77
Figure 4. Western blot images of total protein extracted from snap-frozen samples of liver metastasis from colon adenocarcinoma (LMCA)
from three patients demonstrating the presence of pro-renin receptor (PRR) in all three samples at 22 kDa (A), with the specificity of
the antibody confirmed using a recombinant PRR protein. Angiotensin II receptor 1 was detected in all three samples at the expected
molecular weight of 41 kDa (B); angiotensin II receptor 2 was detected in two out of three samples at 50 kDa (C); angiotensin converting
enzyme was detected at a low abundance in all three samples at the expected molecular weight of 194 kDa (D); and β-actin confirmed
approximate equivalent protein loading for all LMCA samples examined (E)
PTS suggests a role in regulating epithelial-to-mesenchymal transition by influencing angiogenesis [16,24] ,
although this requires further investigation.
