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itself. Second, 5 identified  “HCC  specific” miRNAs   to 2005. J Clin Oncol 2009;27:1485-91.
            were all down-regulated in tumor tissue,  which    4.   Siegel RL, Miller KD, Jemal A. Cancer statistics, 2015. CA Cancer J
                                                                  Clin 2015;65:5-29.
            requires  more sensitive  methods of detection for   5.   El Serag HB, Rudolph KL. Hepatocellular carcinoma: epidemiology
            future clinical application. It is known that RNA-seq   and molecular carcinogenesis. Gastroenterology 2007;132:2557-76.
                                             [61]
            has a better sensitivity than RT-qPCR,  but the latter   6.   Cabibbo G, Maida M, Genco C, Antonucci M, Camma C. Causes of
                                                                  and prevention strategies for hepatocellular carcinoma. Semin Oncol
            is more accurate and usually used for the validation   2012;39:374-83.
            of candidate miRNAs.  We also observed that the    7.   Wogan GN. Aflatoxins as risk factors for hepatocellular carcinoma in
                                [62]
            changes of miRNAs in tumor tissue detected by RT-  8.   humans. Cancer Res 1992;52:s2114-8.
                                                                  Li J, Shi W, Gao Y, Yang B, Jing X, Shan S, Wang Y, Du Z. Analysis
            qPCR  were  minor  compared  to those  by  RNA-seq    of microRNA expression profiles in human hepatitis B virus-related
            [Supplementary Table 3]. Even more challenging     9.   hepatocellular carcinoma. Clin Lab 2013;59:1009-15.
                                                                  Park KU, Seo YS, Lee YH, Park J, Hwang I, Kang KJ, Nam J, Kim
            is  to measure these  miRNAs in circulation in  pre-  SW, Kim JY. Altered microRNA expression profile in hepatitis B virus-
            diagnostic samples, which strongly suggests a dire    related hepatocellular carcinoma. Gene 2015;573:278-84.
            need for development of more sensitive PCR-based   10.  Diaz G, Melis M, Tice A, Kleiner DE, Mishra L, Zamboni F, Farci P.
                                                                  Identification of microRNAs specifically expressed in hepatitis C virus-
            assays that can be used in large population studies.  associated hepatocellular carcinoma. Int J Cancer 2013;133:816-24.
                                                               11.  Edge SB, Compton CC. The American Joint Committee on Cancer: the
            In conclusion, our study identified 33 miRNAs         7th edition of the AJCC cancer staging manual and the future of TNM.
                                                                  Ann Surg Oncol 2010;17:1471-4.
            significantly aberrantly expressed in HCC tumors with   12.  Chu A, Robertson G, Brooks D, Mungall AJ, Birol I, Coope R, Ma
            over 2-fold changes, and for the first time distinguished   Y, Jones S, Marra MA. Large-scale profiling of microRNAs for The
                                                                  Cancer Genome Atlas. Nucleic Acids Res 2016;44:e3.
            5 of them as having “HCC tumor type specificity”,   13.  Barrett T, Troup DB, Wilhite SE, Ledoux P, Rudnev D, Evangelista
            while another 8 are “tumor common” alterations.       C,  Kim  IF,  Soboleva A,  Tomashevsky  M,  Marshall  KA,  Phillippy
            We also found several etiology-related miRNA panels,   KH, Sherman PM, Muertter RN, Muertter RN. NCBI GEO: archive
            but most overlap with those observed in overall HCC.   for high-throughput functional genomic data.  Nucleic Acids  Res
                                                                  2009;37:D885-90.
            These findings have promising applications to better   14.  Schmittgen  TD, Livak KJ.  Analyzing real-time PCR data by the
                                                                  comparative C(T) method. Nat Protoc 2008;3:1101-8.
            understand the common mechanisms underline         15.  Eminaga S, Christodoulou DC,  Vigneault F, Church GM, Seidman
            tumorigenesis and improve precision prevention        JG.  Quantification  of  microRNA  expression  with  next-generation
            and therapy for specific cancers by targeting tumor   sequencing. Curr Protoc Mol Biol 2013;Chapter 4:Unit.
            specific miRNAs. Large retrospective and prospective   16.  Rice J, Roberts H, Burton J, Pan J, States V, Rai SN, Galandiuk S.
                                                                  Assay reproducibility in clinical studies of plasma miRNA. PLoS One
            studies to evaluate miRNA changes in circulation and   2015;10:e0121948.
            trends during cancer development are warranted.    17.  Tibshirani R, Hastie T, Narasimhan B, Chu G. Diagnosis of multiple
                                                                  cancer types by shrunken centroids of gene expression. Proc Natl Acad
                                                                  Sci U S A 2002;99:6567-72.
            Acknowledgments                                    18.  Simon R, Lam A, Li MC, Ngan M, Menenzes S, Zhao Y. Analysis
            We used public available miRNAs and clinical  data    of  gene  expression  data  using  BRB-ArrayTools.  Cancer  Inform
                                                                  2007;3:11-7.
            from TCGA in this manuscript. Information about    19.  Lu TP, Lee CY, Tsai MH, Chiu YC, Hsiao CK, Lai LC, Chuang EY.
            TCGA and the investigators and institutions  that     miRSystem: an integrated system for characterizing enriched functions
            constitute the TCGA research network can be found     and pathways of microRNA targets. PLoS One 2012;7:e42390.
            at http://cancergenome.nih.gov.                    20.  Chen J, Bardes EE, Aronow BJ, Jegga AG. ToppGene Suite for gene
                                                                  list enrichment analysis and candidate gene prioritization.  Nucleic
                                                                  Acids Res 2009;37:W305-11.
            Financial support and sponsorship                  21.  Lee L, Wang K, Li G, Xie Z, Wang Y, Xu J, Sun S, Pocalyko D, Bhak
            This work is supported by NIH grants R01 ES005116,    J, Kim C, Lee KH, Jang YJ, Yeom YI, Yoo HS, Hwang S. Liverome:
            P30 ES009089, P30 CA013696, R03 CA156629. The         a curated database of liver cancer-related gene signatures with self-
                                                                  contained context information. BMC Genomics 2011;12 Suppl 3:S3.
            Cancer Genome Atlas (TCGA) project is supported by   22.  Hou W, Bonkovsky HL. Non-coding RNAs in hepatitis C-induced
            the NCI and NHGRI.                                    hepatocellular  carcinoma:  dysregulation  and  implications  for
                                                                  early detection,  diagnosis and therapy.  World J Gastroenterol
                                                                  2013;19:7836-45.
            Conflicts of interest                              23.  Pogribny IP, Rusyn I. Role of epigenetic aberrations in the development
            There are no conflicts of interest.                   and progression of human hepatocellular carcinoma.  Cancer Lett
                                                                  2014;342:223-30.
                                                               24.  Salvi  A,  Abeni E, Portolani N, Barlati S, De Petro G. Human
            REFERENCES                                            hepatocellular carcinoma cell-specific miRNAs reveal the differential
                                                                  expression of miR-24 and miR-27a in cirrhotic/non-cirrhotic HCC. Int
            1.   Calin GA, Croce CM. MicroRNA signatures in human cancers. Nat   J Oncol 2013;42:391-402.
               Rev Cancer 2006;6:857-66.                       25.  Li B, Huang P, Qiu J, Liao Y, Hong J, Yuan Y. MicroRNA-130a is
            2.   Lyra-Gonzalez I, Flores-Fong LE, Gonzalez-Garcia I, Medina-  down-regulated in hepatocellular carcinoma and associates with poor
               Preciado D,  Armendariz-Borunda J. MicroRNAs dysregulation in   prognosis. Med Oncol 2014;31:230.
               hepatocellular carcinoma: insights  in  genomic medicine.  World J   26.  Zhang X, Daucher M, Armistead D, Russell R, Kottilil S. MicroRNA
               Hepatol 2015;7:1530-40.                            expression profiling in HCV-infected human hepatoma cells identifies
            3.   Altekruse SF, McGlynn KA, Reichman ME. Hepatocellular carcinoma   potential anti-viral targets induced by interferon-alpha.  PLoS One
               incidence, mortality, and survival trends in the United States from 1975   2013;8:e55733.


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