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Venkatesh et al. Cancer Drug Resist 2021;4:223-32 I http://dx.doi.org/10.20517/cdr.2020.84 Page 231

Figure 3. Quantitation of DUPA-FRET fluorescence change upon internationalization in LNCaP cells. Red and green channel fluorescence
intensity was quantitated and the percentage of the red compared to total fluorescence was determined and plotted. Error bars
represent standard deviation. FRET: fluorescence resonance energy transfer

conclude that at least one pathway includes a reducing endosome, while at least one other pathway does
[11]
not. Because the folate trafficking pathway examined earlier results in quantitative disulfide reduction,
a second conclusion must be that not all internalizing receptors traffic through identical intracellular
compartments. For ligand-targeted drug delivery, the obvious third conclusion is that the intracellular
trafficking pathways that process different ligand-targeted receptors must be characterized before a redox
catalyzed cleavage mechanism should be exploited for intracellular drug release.

DECLARATIONS
Authors’ contributions
Made substantial contributions to the conception and design of this study: Low PS
Performed compound synthesis and data acquisition: Venkatesh C, Shen J
Analyzed data: Venkatesh C, Shen J, Putt KS
Wrote manuscript: Putt KS, Low PS

Availability of data and materials
All data source could be available to readers on request.

Financial support and sponsorship
This work was supported by a grant from Endocyte.

Conflicts of interest
All authors declared that there are no conflicts of interest.

Ethical approval and consent to participate
Not applicable.