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Citterio et al. Plast Aesthet Res 2020;7:41 I http://dx.doi.org/10.20517/2347-9264.2020.29 Page 7 of 20

No differences were found between autologous and allogenic groups. a) and c) showed collagen fibers oblique and parallel to the root. b) had perpendicularly inserted fibers in new cementum PL regeneration was found in both groups Test group showed higher cementum, associated with higher attachment gains. No increased bone regeneration was found Group with PDLC showed more cementum, bone and ligament formation No differences in bone

-Histological: Azan staining -Enzyme-linked immunosorbent assay: CRP, IL-10, IFN-c, CD30 -Histomorphometric -Radiological: micro-CT -Histological: HES, Masson’s trichrome -Immunohistochemical: Col1 -Histological: Toluidine blue staining -Histomorphometric percentages of new -Histological: HES, Masson trichrome staining -Histomorphometric -Histological: HES, Masson’s trichrome staining -Histomorphometric -Histological: HES, Azan

(a) PDLSC autologous/PGA + 2 months β-TCP + collagen (b) PDLSC allogenic/PGA + β-TCP + collagen (c) β-TCP + collagen 3 months (a) jBMSC + PDLSC + TDM + (b) iBMSC + PDLSC + TDM + 3 months (a) CDC + collagen (b) PDLSC + collage (c) collagen 2 months (a) PDLC + hyaluronic acid (b) hyaluronic acid sheet 1 month (a) PDLSC + atecollagen (b) empty 1, 5 months (a) PDLC + PGA + β-TCP (b) PGA + β-TCP 3 months (a) PDLSC + HA-β-TCP (b)

Intrabony defects 5 mm × 5 mm Intrabony CA defects 5.2 mm × 5 mm CA Intrabony defects 3 mm × 4 mm Dehiscence sheet defects 5 mm × 5 mm Fenestration defects 6 mm × 4 mm Intrabony defects 5 mm × 5 mm × 4 mm Intrabony defects 7 mm × 3 mm × 5 mm Intrabony defects 4 mm × 5 mm Intrabony defects 3 mm × 5 mm × 8 mm

8 (a, b, c) 10 (a) 8 (b) 8 (a, b, c) 5 (a, b) 6 (a, b) 4 (a, b) 12 (b, c) 5 (unclear) 10 (a, b)

DOG Beagle MINIATURE PIG DOG Beagle male 1 year old DOG Beagle female 3 years-old DOG Beagle female DOG Beagle male MINIATURE PIG 24 (a) 12 months old DOG 18-36 months old DOG Mongrel 1-2 years old

PGA/β-TCP/ collagen and absorbable membrane (GTR) Treated dentine matrix 2 years old (TDM) and ceramic bone (CA) Collagen Hyaluronic acid sheet type I (70- 80%) and type III (20- 30%) AC + ePTFE membranes PGA/β-TCP HA/β-TCP + gelatin membranes AC 3-4 Demineralized male bovine bone matrix granules

PDLSC PDLSC BMSC CDC PDLSC PDLC PDLSC PDLC PDLSC PDLC DPSC

Tsumanuma et al. [88] 2016 Zhu et al. [89] 2017 Nuñez et al. [90] 2012 Akizuki et al. [95] 2005 Nakahara et al. [96] 2004 Iwata et al. [97] 2009 Liu Y et al. [138] 2008 Inukai et al. [98] 2013 Khorsand et al. [67] 2013