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Ohashi. Plast Aesthet Res 2020;7:26 I http://dx.doi.org/10.20517/2347-9264.2020.15 Page 5 of 10

Table 2. Characteristics of patients who received their cryopreserved fat (n = 216)
Duration Aug 2015 ~ Dec 2018
Sex Male 14 Female 202
Age (years) 19-79 (40.8 ± 11.5)
Height (cm) 147.5-188 (160.0 ± 5.9)
Weight (kg) 38.5-90.7 (52.1 ± 8.1)
2
Body mass index (kg/m ) 15.9-31.6 (20.3 ± 2.5)
Table 3. Number of patients with different injection times using cryopreserved fat from one harvesting

1 time 216 patients
2 times 59 patients
3 times 25 patients
4 times 10 patients
5 times 1 patient
6 times 1 patient

Table 4. The ways cryopreserved fat was used
Volume augmentation only - 169 cases
Facial rejuvenation 143 cases
Body augmentation 26 cases
Revitalization/fertilization only - 98 cases
Improve skin condition only 11 cases
Scar only 55 cases
Improve skin condition and for scar 32 cases
Volume augmentation + revitalization/fertilization 87 cases
(Treatment for scar & fibrous tissue with/without PALF)
Face (Injury scar, revision of liposuction, acne scar) 53 cases
Revision of liposuction (thigh, abdomen) 19 cases
Revision of SIEF 5 cases
Incision scar (IMF, nipple, axillar) 10 cases
SIEF: simultaneous implant exchange with fat; PALF: percutaneous
aponeurotomy and lipofilling

Follow-up of patients
We followed-up each patient after at one month and 3-6 months after the first injection using fresh fat, and
also at one month and 3-6 months after repeat injections using thawed cryopreserved fat.

Comparison of stromal vascular fraction
We compared the amount of stromal vascular fraction (SVF) in fresh fat before sending it to the CPC
with the SVF of thawed cryopreserved fat. SVF was digested by collagenase (Wako Pure Chemical, Osaka,
Japan). Cell count and viability of SVF were performed by KUNA-STEAM Automated Fluorescence Cell
Counter (Logos Biosystems, South Korea).

RESULTS
Of the 490 patients who underwent fat harvesting in our clinic, 216 patients (312 cases) received fat grafting
with cryopreserved fat. The characteristics of those patients are shown in Table 2. The number of patients who
received a varying number of injections from one harvesting session is shown in Table 3. The ways of using
cryopreserved fat are shown in Table 4. The injected volume of cryopreserved fat was from 0.2 to 24.0 mL
for the face and from 4.0 to 100.0 mL for the body.

There were no severe complications in any patients. Mild complications occurred in 5 patients (2.3%), all of
which were temporary pigmentation [Table 5].