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Ohashi. Plast Aesthet Res 2020;7:26 I http://dx.doi.org/10.20517/2347-9264.2020.15 Page 3 of 10

Table 1. Characteristics of patients who had their fat cryopreserved (n = 490)
Duration Aug 2015 ~ Dec 2018
Sex Male 26 Female 464
Age (years) 19-81 (40.9 ± 11.6)
Height (cm) 142-188 (160.0 ± 5.8)
Weight (kg) 37.6-90.7 (52.1 ± 8.0)
2
Body mass index (kg/m ) 15.0-33.5 (20.3 ± 2.5)

Figure 1. Flowchart of cryopreservation and fat grafting. This figure is used with permission from Ohashi published in Clin Plast Surg [16]

The main injection sites for volume augmentation are the breasts and the face (such as the forehead and
cheeks). For skin rejuvenation, the main injection sites are the lower eyelids, the area surrounding the lips,
and areas with signs of facial aging, e.g., wrinkles. Sometimes fat grafting is used for scar treatment such as
in cases of double eyelids, liposuction revision, or contracture release in percutaneous aponeurotomy (so-
called rigottomy).

How to send the fat
After fat is utilized on the day of harvest, the remaining fat is collected in a bag (FB-bag: CellSource Co.)
which is then packed in an ADIPORTER box (CellSource Co., Ltd., Tokyo, Japan). After packing, the bag is
sent to the CPC at CellSource Co. in a refrigerated state (below 10 °C) [Figure 2].

Cryopreservation and storage of fat
The process of cryopreservation and storage at -196 °C was performed in the CPC at CellSource Co.

The details of this process are confidential matters for the company. Roughly speaking, delivered fat is
washed using Ringer’s lactate solution. The fat is mixed with a cryoprotectant and divided into 4- to 5-mL
aliquots in syringes. These syringes are gradually frozen at a controlled rate until the temperature reaches