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Page 4 of 10 Ohashi. Plast Aesthet Res 2020;7:26 I http://dx.doi.org/10.20517/2347-9264.2020.15

A B

Figure 2. Kit for transportation. A: FB-bag (CellSource Co., Ltd., Tokyo, Japan), which contains an adipose tissue transport medium;
B: Adiporter (CellSource Co., Ltd., Tokyo, Japan), which is the box in which the fat is sent to the cell processing center company in a
refrigerated state (below 10 °C). This figure is used with permission from Ohashi et al. [18] published in Plast Reconstr Surg Glob Open

Figure 3. Thawed cryopreserved fat. This photograph was taken at our clinic. The material looks like fresh fat, and there is almost no oil
in those syringes. This figure is used with permission from Ohashi et al. [18] published in Plast Reconstr Surg Glob Open

-80 °C. They are then transferred to liquid nitrogen and stored at -196 °C.

Using this process, we can store many syringes of patients’ fat after single harvesting sessions.

Return of fat
When we want to use cryopreserved fat, we use an Internet web-ordering service from the CellSource CPC
to place an order. After the CPC receives the order, the patient’s cryopreserved fat is thawed rapidly (37 °C)
and the cryoprotectant liquid is washed out. The CPC sends back that fat to our clinic in a refrigerated state
(below 10 °C) [Figure 3].

Injection of thawed cryopreserved fat
The thawed fat must be injected within 48 hours of receiving it. We follow the “Coleman technique” for
volume augmentation, and use the “nanofat (emulsified fat) technique” for skin rejuvenation. The fat is
usually used at multiple injection sites. For example, the first injection of cryopreserved fat may be used for
breast augmentation and the second injection for forehead volume augmentation and/or scar treatment.
We may use the cryopreserved fat many times if the supply lasts. Therefore, we can inject each patients
many times easily.