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Tang et al. Neuroimmunol Neuroinflammation 2021;8:64-9 I http://dx.doi.org/10.20517/2347-8659.2020.28 Page 67

Figure 2. Efficacy of tumor resection shown by HE staining. Mice received tumor cells (i.c.) on day 0, and tumor resection occurred on
day 16. Mice were euthanized on day 17, followed by overnight incubation of tissue sections in 95% ethanol and standard HE staining
protocol. Representative HE stained sections are shown. Pink: normal brain tissue; dark blue: tumor. Scale bar = 2.5 mm, n = 2 for both
groups

RESULTS
Resection of tumor on live mice is feasible and has the potential to prolong survival for mice
bearing advanced glioma
A technique was developed to perform tumor resection on live mice bearing advanced glioma in the
4
brain. Glioma tumors were established by direct intracranial injection of 5 × 10 GL261 NS cells/mouse
into the right striatum. Tumor resection was performed 16 days after tumor implantation. Four mice
were euthanized on day 17 for HE staining, and the remaining four mice were reserved for the survival
experiment. Figure 2 shows that the advanced glioma occupied about 25% of area of the right hemisphere
of the brain and that surgical resection removed approximately 85% of the glioma tumor. The survival
curve [Figure 3] shows that the median survival for resected mice was prolonged by 5 days (although short
of significant difference) compared to non-resected mice. These results indicate that it is feasible to perform
tumor resection on mice bearing advanced glioma in the brain, that mice are able to survive the resection,
and that surgical resection has the potential to prolong survival time.