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Neuroimmunol Neuroinflammation 2018;5:37 Neuroimmunology and
DOI: 10.20517/2347-8659.2018.45 Neuroinflammation

Meeting Abstracts Open Access

2018 Mental Health and Neurology Conference

Bangkok, Thailand; Sep 2018; Published: 25 Sep 2018
Correspondence to: Dr. Moh Hasan Machfoed, Department of Neurology, Medical Faculty of Airlangga University, Surabaya 60115,
Indonesia. E-mail: mh.machfoed@gmail.com

1. Evaluation of hypermethylation and expression pattern of glutamate and dopamine
receptors genes in patients with schizophrenia

Dor Mohammad Kordi Tamandani

University of Sistan and Baluchestan

Aim: Schizophrenia (SCZ) is a type of psychotic disorders that affects 1% population. Dopamine and
glutamate are the major neurotransmitters in brain and their receptors are associated with the number of
psychotic disorders such as schizophrenia. The aims of the present study were to analyze methylation and
expression profile of dopamine and glutamate receptors genes in patients with SCZ.

Methods: Methylation-specific polymerase chain reaction (MS-PCR) was used to estimate promoter
hypermethylation of dopamine and glutamate receptors genes on 81 isolated genomic DNA, from peripheral
blood of individuals with schizophrenia and 71 healthy control subjects. In addition, real-time reverse
transcription - PCR was used to estimate mRNA levels in 34 blood samples of healthy controls and cases.

Results: Methylation of GRM2 and GRM5, highly increased the risk of schizophrenia in comparison to
reference unmethylated pattern [OR = 2.82, (1.05-7.75), P = 0.038], [OR = 12.09, (1.84-79.57), P = 0.0001]
respectively. Regarding the dopamine receptors genes, promoter methylation of DRD4 and DRD5 genes were
statistically different (P < 0.05) in cases when compared with healthy controls in blood samples. Outcomes of
expression analysis revealed statically significant difference between cases (n = 17) and health controls (n =
17) regarding relative genes expression of GRM2, GRM5 and GRIA3, DRD2, DRD4 and DRD5 (P < 0.0001).

Conclusion: To the best of our knowledge, this is the first report which indicates the methylation status and
expression profile of GRs and DRs genes with the risk of SCZ. These outcomes suggested more attention to
the effect of epigenetic variations in development of SCZ in further investigations.

© The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0
International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use,
sharing, adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as long
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