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Page 107 Chu et al. J Transl Genet Genom 2023;7:196-212 https://dx.doi.org/10.20517/jtgg.2023.22
Figure 4. Performance statistics of 240 genome sequencing (GS) conducted by HKGI. (A) Quality indicators and thresholds used in
monitoring the quality of different stages in the GS workflow. (B) Plots showing quality and quantity statistics of the 240 extracted
gDNA. (C) Plots showing quality and quantity statistics of the 240 GS libraries. (D) Performance statistics of 240 GS libraries in ten
NovaSeq 6000 runs. The 240 samples all meet the stringent quality indicators and metrics for assessing the quality of the samples and
sequencing libraries.
(TapeStation, Agilent). Amongst the 240 GS libraries shown, the library insert size ranges from 400 to
700 bp, with a median insert size of 496 bp, which is within the range of efficient cluster formation on the
Illumina patterned flow cells. GS libraries that pass the above quality controls then proceed to qPCR
