Page 109 - Read Online

Page 4 of 12 Schofield et al. J Cancer Metastasis Treat 2020;6:10 I http://dx.doi.org/10.20517/2394-4722.2019.43

Table 1. The expression of a-enolase is altered in cancer
Alteration (frequency of
Cancer Sample type Detection method Ref.
alteration)
AML Peripheral blood and bone marrow from AML Microarray Increased [4]
patients (n = 41) and healthy patients (n = 20)
Breast cancer Breast cancer tissue with adjacent matched Western blot Increased (100%) [9]
normal tissue (n = 24)
Breast cancer and normal tissue (n = 244) Quantitative real-time PCR Decreased [10]
Cervical Squamous cell carcinoma (n = 33) and normal 2D-DIGE and MALDI-TOF Increased [40]
cancer cervical tissue (n = 17) mass spectrometry
Colorectal SW620 cell line (lymph node metastasis) 2D-DIGE Increased [41]
cancer compared to SW480 (primary lesion)
Colorectal cancer (n = 48) and adjacent matched IHC and PCR Increased (56%) [11]
normal tissue (n = 16)
Endometrial Endometrial cancer (n = 100), endometrial IHC Increased in endometrial cancer [12]
carcinoma atypical hyperplasia (n = 22) and normal (52%) and atypical hyperplasia
endometrium tissues (n = 20) (31.8%) compared to normal
Gastric Gastric cancer and adjacent normal tissue (n = IHC Increased [13]
cancer 94)
Primary gastric cancer tissue (n = 107) IHC Increased (48%) [14]
Glioma Primary glioblastoma tissue (n = 24) Quantitative real-time PCR Increased (68%) [5]
Glioma (n = 136) and normal brain (n = 15) tissue Quantitative real-time PCR Increased (69%) [6]
and IHC
Head and Head and neck cancer and adjacent normal Real-time PCR Increased (50%) [15]
neck cancer tissue (n = 44)
HCC HCC (n = 374 and n = 1309), adjacent matched Microarray and IHC Increased [16]
tissue (n = 50), and normal tissue (n = 1442)
from TCGA and GEO data source; tissue
microarray (93 HCC and 87 normal liver tissues)
Lymphoma Pretherapeutic tumour biopsies from peripheral IHC Increased [8]
T-cell lymphoma not otherwise classified (n =
87)
Melanoma A375, MeWo, MEL-HO, Colo-800, Colo-853 2D-DIGE and nano- Increased [7]
melanoma cell lines and a normal melanocyte HPLC-chip ion trap mass
cell line spectrometry
NSLC Primary NSCLC tissue and matched normal lung Real-time PCR and IHC Increased [17]
(n = 26) from RNA and primary NSCLC tissue (n
= 55) and normal lung tissue (n = 17)
Primary NSCLC and adjacent matched normal Western blotting Decreased (26%) [42]
tissue (n = 46)
Primary NSCLS tissue (n = 36) PCR Increased (16%) [18]
Ovarian Ovarian cancer (n = 4) and ovarian tissue from 2D-DIGE and LC-MS/MS Increased [19]
cancer endometriosis (n = 1)
Pancreatic Pancreatic cancer and adjacent normal tissue (n IHC Increased [20]
cancer = 31)
Pancreatic cancer (n = 100) and adjacent normal IHC Increased (48%) [21]
tissue (n = 80)
Primary pancreatic and adjacent normal tissue (n Western blot Increased [22]
= 3)
Prostate Exosomes from prostate cancer cell lines Western blot and mass Decreased [43]
cancer spectrometry

2D-DIGE: two-dimensional differential in gel electrophoresis; HCC: hepatocellular carcinoma; HPLC: high performance liquid
chromatography; IHC: immunohistochemistry; LC-MS/MS: liquid chromatography tandem mass spectrometry; MALDI-TOF: matrix
assisted laser desorption/ionization time of flight; NSCLC: non-small cell lung cancer; PCR: polymerase chain reaction; AML: acute
myeloid leukaemia; TCGA: the cancer genome atlas; GEO: gene expression omnibus

and exhibit an increased expression of proteins involved in both oxidative stress- and sirtuin-induced
[62]
autophagy . Taken together, these studies demonstrate that a-enolase is an important regulator of tumour
cell metabolism, proliferation and survival, which by definition make it a perfect target for anticancer
therapy.