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Page 8 of 20 Ottewell et al. J Cancer Metastasis Treat 2021;7:11 https://dx.doi.org/10.20517/2394-4722.2021.14
Figure 1. Effects of oestradiol on mouse tibiae. Eight-week-old BALB/c nu/nu mice received a 28-day release 1.7 mg 17 oestradiol pellet
via subcutaneous implant or 12 mg/L 17 oestradiol continually via their drinking water (n = 10/group). Mice were culled after 28 days
and serum and tibiae isolated for analysis: (A) mean ± SEM circulating concentrations of 17 oestradiol as measured by ELISA; (B)
effects of 17 oestradiol on mean ± SEM for percentage of trabecular bone volume compared with tissue volume (BV/TV%) in the
proximal tibiae; (C) representative, three-dimensional micro-CT images of tibiae from control (untreated) and 17 -treated tibiae.
Statistical analysis was by student t-test. **P < 0.01; ***P < 0.001. Data shown are previously unpublished observations from the
Ottewell laboratory.
results in development of overt metastases in 80%-90% of adult mice even after long-term dormancy [42,43] .
