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Page 4 of 20 Ottewell et al. J Cancer Metastasis Treat 2021;7:11 https://dx.doi.org/10.20517/2394-4722.2021.14

Table 1. Summary of immune compromised mouse models of breast cancer bone metastasis
Cell line Origin Sub-line Model system Inoculation route Metastatic site Lesion
MDA- Human Parental BALB/c nude Intra-cardiac Mouse long bones, Osteolytic 2-3 weeks
MB-231 mammary (ER-PR-HER2-) MF1 nude spine and jaw
adenocarcinoma NOD SCID Intra-tibial Mouse tibiae Osteolytic 1-2 weeks
isolated from a NSG
pleural effusion Orthotopic (MIND) Human bone x-plants Osteolytic 4-7 weeks
MDA-MB-231- BALB/c nude Intra-cardiac Mouse long bones, Osteolytic 2-3 weeks
BO2 Intra-arterial spine and jaw
(ER-PR-HER2-)
Intra-tibial Mouse tibiae Osteolytic 1-2 weeks
MDA-MB-231-IV BALB/c nude Intra-cardiac Mouse long bones, Osteolytic 2-3 weeks
(ER-PR-HER2-) Intra-arterial spine
Intra-venous
NOD SCID Orthotopic (MIND) Human bone X-plants Osteolytic 4-7 weeks
MDA- Human Parental MF1 nude Intra-tibial Mouse tibiae Osteolytic 2-3 weeks
MB-436 mammary (ER-PR-HER2-) BALB/c nude
adenocarcinoma NOD SCID
isolated from a
pleural effusion
SUM- Isolated from a Parental NOD SCID Orthotopic (fat pad) Human bone X-plants Osteolytic 4-8 weeks
1315 metastatic (ER-PR-HER2-) Intra-tibial Mouse tibiae Osteolytic 3-4 weeks
nodule from
ductal
carcinoma
MCF-7 Human Parental BALB/c nude Intra-cardiac Mouse long bones Mixed 20-25 weeks
mammary (ER+PR+HER2-) Intra-tibial Mouse tibiae Mixed 1-3 weeks
adenocarcinoma under estradiol
isolated from a supplementation Orthotopic (fat pad) Mouse long bones Mixed 4-6 weeks
pleural effusion
NOD SCID Orthotopic (fat pad) Mouse long bones Mixed 6-8 weeks
Human X-plants
MCF-7/Neu BALB/c nude Intra cardiac Mouse long bones Mixed 10-12 weeks
Intra tibial Mouse long bones Mixed 1-3 weeks
T47D Human Parental BALB/c nude Intra-cardiac Mouse long bones Mixed 20-25 weeks
mammary (ER+PR+HER2+) Intra-tibial Mouse tibiae Mixed 1-3 weeks
ductal under estradiol
carcinoma supplementation) Orthotopic (fat pad) Mouse long bones Mixed 4-6 weeks
isolated from a NOD SCID Orthotopic (fat pad) Mouse long bones Mixed 6-8 weeks
pleural effusion
Human X-plants
ZR75-1 Human ductal Parental BALB/c nude Intra-cardiac Mouse long bones and Mixed 12-25 weeks
carcinoma (ER+PR+ER2+) spine
derived from a
malignant
ascitic effusion
Patient Human ductal BB2RC08 NSG Orthotopic (MIND) Mouse long bones, Lytic 14+ weeks
derived carcinoma (ER+PR+HER2-) lungs and human bone
xenograft created from Under estradiol X-plants
(PDX) primary breast supplementation Intra-cardiac Mouse long bones, Lytic 14+ weeks
tumour
lungs and human bone
X-plants
Human ductal BB3RC32 NSG Orthotopic (MIND) Mouse long bones, Lytic 15+ weeks
carcinoma (ER+PR+HER2-) lungs and human bone
isolated from Under estradiol X-plants
ascitic fluid supplementation
Intra-cardiac Mouse long bones, Lytic 15+ weeks
lungs and human bone
X-plants
Human ductal BB6RC37 NSG Orthotopic (MIND) Mouse long bones, Lytic 12+ weeks
carcinoma (ER-PR-HER2-) lungs and human bone
created from Under estradiol X-plants
primary breast supplementation Intra-cardiac Mouse long bones, Lytic 15+ weeks
tumour
lungs and human bone
X-plants

short duration between injection of MDA-MB-231 cells and metastatic outgrowth indicates a lack of