Page 31 - Read Online
P. 31

Data in Figure 5b show that AZA treatment induced a   architecture [Figure 6]. The liver sections obtained from
          significant increase in mean relative liver weight (RLW)   FSME and CFME treated rats showed more or less normal
          when compared to normal control rats. However, the oral   hepatocytes architecture, but some congested blood
          administration of CFME or FSME did not significantly affect   vessels were seen in FSME-treated rats [Figure 7] and mild
          the mean RLW. The pre-treatment with CFME did not improve   inflammation around the portal tract in the CFME-treated
          the increase in mean RLW ratio induced by AZA treatment,   rats [Figure 8]. In contrast, the liver sections obtained from the
          but the administration with FSME ameliorated the increase   AZA-treated rats revealed hepatocytes disorganization, and
          in mean RLW induced by AZA treatment.               fatty degeneration as indicated by large and microvesicular
                                                              fat droplets. The hepatocytes nuclei were shrinked and
          The light microscopical examination of the liver sections   pyknotic or apoptotic. There were areas of hemorrhages
          from the control rats revealed normal hepatocytes   in blood vessels and in between hepatocytes. Hepatocytes

















                         a                                    b
















                                              c
          Figure 2: (a) Serum alanine aminotransferase (ALT) activity; (b) aspartate aminotransferase (AST) activity; (c) alkaline phosphatase (ALP) activity in rats treated daily for
          28 consecutive days with chamomile fl owers methanolic extract (CFME), fennel seeds methanolic extract (FSME), azathioprine (AZA), CFME + AZA, and FSME + AZA.
                                                                   †
          Data represent the mean ± standard error (n = 8). *P ≤ 0.05 compared to normal control rats;  P ≤ 0.05 compared to AZA-treated rats


















                         a                                   b
          Figure 3: (a) Serum total-bilirubin (T.BIL) level; (b) direct-bilirubin (D.BIL) in rats treated daily for 28 consecutive days with chamomile fl owers methanolic extract
          (CFME), fennel seeds methanolic extract (FSME), azathioprine (AZA), CFME + AZA, and FSME + AZA. Data represent the mean ± standard error (n = 8). *P ≤ 0.05
          compared to normal control rats;  P ≤ 0.05 compared to AZA-treated rats
                               †

               Hepatoma Research | Volume 1 | Issue 3 | October 15, 2015                                    129
   26   27   28   29   30   31   32   33   34   35   36