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Anea et al. Vessel Plus 2018;2:16 I http://dx.doi.org/10.20517/2574-1209.2018.46 Page 5 of 8
Bmal1 Clock Npas2 Per1 Cry1
12:00 AM
12:00 PM
Rev-erb alpha Ror-alpha Epas CK
12:00 AM
12:00 PM
Figure 2. Circadian clock expression in human saphenous vein. Human saphenous veins that remained from coronary artery bypass were
immediately procured post-operatively, and incubated in an oxygenated 37 °C incubator. At indicated times, sections of the saphenous
vein were frozen in OCT, then sectioned, and incubated with indicated antibodies to the circadian clock
75
Bmal1
50
75
Cry1
37
Gapdh
25
24:00 3:00 6:00 9:00 12:00 15:00 18:00 21:00
0.7
0.6
0.5
Bmal1/Gapdh 0.4
0.3
0.2
0.1
0.0
24:00 3:00 6:00 9:00 12:00 15:00 18:00 21:00
Time of day
1.6
1.4
1.2
Cry1/Gapdh 0.8
1.0
0.6
0.4
0.2
0.0
24:00 3:00 6:00 9:00 12:00 15:00 18:00 21:00
Time of day
Figure 3. Bmal1 and Cry expression in the human saphenous vein. Human saphenous vein was incubated at 37 °C, and sections were
harvested at 3 h intervals from midnight (24:00) to 21:00 (9 PM) for a 21-h time span. At indicated times, saphenous veins were flash
frozen and subsequently protein lysates isolated for western blotting (top panel) that was densitometrically quantified (bottom panels)
