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Page 4 of 8 Anea et al. Vessel Plus 2018;2:16 I http://dx.doi.org/10.20517/2574-1209.2018.46

Carotid Aorta Femoral artery Heart Lung
and vein
A

12:00 AM

12:00 PM

B

12:00 AM

12:00 PM

C

12:00 AM

12:00 PM

D

12:00 AM

12:00 PM

E
12:00 PM

F

12:00 PM

G

12:00 PM

Figure 1. Circadian clock expression in murine cardiovascular tissues. Antibodies to Bmal1 (A), Clock (B), Cry1 (C), Casein kinase (D),
Npas2 (E), Rora (F), and Per1 (G) were incubated with frozen carotid artery, aorta, femoral artery/vein, heart, and lung isolated at
indicated times of the day to assess localization expression