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Page 10 of 19 Wang et al. Microbiome Res Rep. 2025;4:23 https://dx.doi.org/10.20517/mrr.2024.94

Figure 4. Genus-level composition of top 10 fecal microbiome members in mother and infant groups. (A) and (B) Taxonomic differential
abundance analysis (Top four Phylum level). Statistical significance was analyzed using the Wilcoxon test. (A) Based on relative
abundance; (B) Based on the total copy number of Spike-in; (C) Relative abundance based on the percentage of the top 10 genera; (D)
Absolute abundance based on the copy number (per gram of feces) of the top 10 genera by using spike-in. The calculation of the top 10
microbial genera is based on the summation of genus copies from both mothers and infants, sorted accordingly; (E) The calculation of
the top 10 microbial genera is based on the summation of genus copies separately from mothers and infants, and subsequently sorted.