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Page 12 of 19 Misera et al. Microbiome Res Rep 2024;3:48 https://dx.doi.org/10.20517/mrr.2023.81

Table 1. Effects of A. muciniphila and its outer membrane protein Amuc_1100 on anxiety and depression-like behavior in mice

Tested animals Intervention
Model of (age, weight, (dosage, Analytical techniques Samples tested Outcomes Conclusions Limitations Ref.
stress
number, sex) duration)

CRS and DSS- Male C57BL/6N A. muciniphila MucT Behavioral tests (OFT, TST, FST), Serum, colon Behavioral tests: increased distance A. muciniphila supplementation Limited to male Chen
induced colitis mice, 18-20 g, n = (DSM 22959), 1 × ELISA, qRT-PCR, 16S rRNA gene tissue, cecal in OFT, decreased immobility in protects against CRS-induced mice; effects on et al.,
8 [94]
32 (experiment 1) 10 CFU/100 µL, sequencing, RNA sequencing, microbiota, fecal TST and FST gut microbiota dysbiosis, females unknown 2021
and n = 72 oral gavage for histology, immunohistochemistry, samples ELISA: decreased corticosterone, colonic mucosal barrier Mechanisms of
(experiment 2) 14 days PAS/AB staining increased dopamine and serotonin damage, and aggravation of action not fully
in serum colitis elucidated
qRT-PCR: increased BDNF mRNA in
hippocampus
16S rRNA gene sequencing: altered
gut microbiota composition
RNA sequencing: identified DEGs
related to gut microbiota and
colonic mucus
Histology: improved colonic
structure
Immunohistochemistry: increased
MUC2 expression
PAS/AB staining: increased number
of goblet cells
CRS Male C57BL/6 A. muciniphila Behavioral tests (OFT, FST, TST), Serum, Behavioral tests: increased distance AKK ameliorates depressive- Limited to male Ding
mice, 6-8 weeks, (ATCC® BAA- ELISA, qRT-PCR, 16S rRNA gene hippocampus in OFT, decreased immobility in like behavior by regulating gut mice; female et al.,
8 [95]
n = 6 per group 835™) (1 × 10 sequencing, untargeted tissue, cecal FST and TST microbiota, metabolites and response unknown 2021
CFU/µL), daily for metabolomics microbiota ELISA: decreased corticosterone, increasing BDNF levels Short duration of
3 weeks increased dopamine and serotonin intervention
in serum
qRT-PCR: increased BDNF mRNA in
hippocampus
16S rRNA gene sequencing: altered
gut microbiota composition
Untargeted metabolomics:
identified 23 potential biomarkers
in serum
CUMS Male C57BL/6 Amuc_1100, 80 µg/ Behavioral tests (OFT, TST, FST), Serum, colon Behavioral tests: increased distance Amuc_1100 improves The study was only Cheng
mice, 5-6 weeks, 200µL, daily for 6 ELISA, qRT-PCR, 16S rRNA gene tissue, brain in OFT, decreased immobility in depression-like behaviors, conducted on male et al.,
[96]
n = 4-8 per group weeks sequencing, (hippocampus) TST and FST increases 5-HT levels in CNS mice; the effects on 2021
immunohistochemistry tissue, fecal ELISA: increased 5-HT in serum and and periphery, modulates gut female mice were
samples colon, decreased corticosterone microbiota, and upregulates unknown
qRT-PCR: increased Tph1 in colon, BDNF and CREB1 in the Mechanisms of
increased BDNF, CREB1, 5-HTR1A hippocampus MGBA interaction
in hippocampus not fully elucidated
16S rRNA gene sequencing: Limited sample size
improved gut microbiota diversity for some tests
and composition Short duration of