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Yakovleva et al. Microbiome Res Rep 2024;3:19 https://dx.doi.org/10.20517/mrr.2023.56 Page 11 of 18

Table 2. The coefficient of biocenotic similarity for the flies and the corresponding substrate
NaCl concentration, Fly and substrate Coefficient of biocenotic similarity. Coefficient of biocenotic similarity.
% samples Bacteria Yeasts *
0 0b and s0b 0.48 0
0c and s0c 0.26 0
2 2a and s2a 0.32 0.74
4 4a and s4a 0.98 0.33
7 7a and s7a 0.25 0.09

* [44]
Estimated based on data from the paper of Dmitrieva et al. .

Table 3. Pairwise Spearman correlations calculated for 11 fly samples
Variables Salt concentration Bacterial abundance Yeast abundance Bacterial diversity Yeast diversity
Salt concentration 1
Bacterial abundance +0.30 1
Yeast abundance +0.33 -0.25 1
Bacterial diversity -0.20 +0.04 -0.66 * 1
Yeast diversity +0.69 * -0.32 +0.47 -0.47 1

*
5% significance level.

Figure 3. Diversity assessment (Shannon index) for the bacterial (diamonds) and yeast (triangles) components of microbial
communities: (A) flies; (B) flies and corresponding substrates. Designation of the fly lines is the same as in Figure 1.

Considering that the microbiomes of the flies reared on the substrate with 7% NaCl concentration (7a-7b)
were contrastingly different from all the others, the calculations were repeated for 9 samples, excluding
these two. In this case, five pairwise correlations out of 10 possible were significant at the 5% significance
level: salt concentration - yeast diversity (positive correlation) as in the case with 11 samples; salt
concentration - yeast abundance (positive correlation), salt concentration - bacterial diversity (negative
correlation), yeast abundance - yeast diversity (positive correlation), bacterial diversity - yeast diversity
(negative correlation). The pair yeast abundance - bacteria diversity (negative correlation), which was
significant at a 5% significance level on a set of 11 samples, became nonsignificant (significant only at a 10%
significance level) on a set of 9 samples [Table 4].