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Yakovleva et al. Microbiome Res Rep 2024;3:19 https://dx.doi.org/10.20517/mrr.2023.56 Page 9 of 18

Table 1. The abundance of bacteria and yeasts in the fly lines adapted to the substrate with different salinity and in the
corresponding substrates
NaCl Fly or substrate Total number of bacteria, CFU per fly or 1 Total number of yeasts, CFU per fly, or 1
concentration, % sample mg of substrate ± standard deviation * mg of substrate ± standard deviation **,***
0 0a 895,000 ± 161,142 3,480 ± 972
0b 2,860,000 ± 245,085 560 ± 246
0c 7,585,000 ± 2,741,660 0
s0b 97,700,000 ± 8,808,140 20 ± 63
s0c 13,021,000 ± 1,606,238 1,020 ± 670
2 2a 47,200 ± 11,013 96,740 ± 10,688
2b 9,900 ± 2,094 191,160 ± 60,733
2c 20,100 ± 2,051 295,300 ± 83,388
s2a 151,400 ± 52,244 118,960 ± 20,188
4 4a 2,107,500 ± 229,982 133,400 ± 32,146
4b 21,840,000 ± 2,633,755 139,120 ± 41,235
4c 6,005,000 ± 599,138 504,440 ± 149,799
s4a 4,492,500 ± 803,632 201,240 ± 59,948
7 7a 8,295,300 ± 957,967 52,306 ± 12,370
7b 1,390,400 ± 422,926 2,068 ± 1,029
s7a 3,100 ± 354 29,596 ± 11,460

* ** ***
30 flies or 3 mL of the substrate, three dilutions, three replicates each; 30 flies or 3 mL of the substrate, ten replicates; the data from the paper
[44]
of Dmitrieva et al. .

Figure 2. The effect of NaCl on the growth rates of dominant species of bacteria associated with flies. Bars represented the average
optical density ratio of a culture grown on a medium with NaCl to that grown on a medium without NaCl (n = 4). The difference between
all groups is statistically significant, P < 0.0005.