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Spus et al. Microbiome Res Rep 2023;2:33 https://dx.doi.org/10.20517/mrr.2023.20 Page 9 of 15

Figure 4. Susceptibility of the single colony isolates from the end time point of the propagation experiment to the three lactococcal
phages used in the cocktail: ϕTIFN1 (ϕ1), ϕTIFN5 (ϕ5), ϕTIFN7 (ϕ7) and to the phages present in the supernatant of the blends at the
end time point (538 generations). Replicates A-1 and A-2 were used as control - no phage cocktail was added. Replicates B-1 and B-2
were challenged at the onset of the experiment with a cocktail of three lactococcal phages: ϕTIFN1, ϕTIFN5 and ϕTIFN7. The strains 1.01
to 1.50 were isolated from blend A-1, strains 2.01 to 2.50 were isolated from blend A-2, strains 3.01 to 3.50 were isolated from blend B-1
and strains 4.01 to 4.50 were isolated from blend B-2 (for a detailed description see Materials and Methods section).

DISCUSSION
This study provides data on lytic bacteriophages affecting the composition of microbial communities with
genetic diversity at the level of genetic lineages. The compositional changes in the microbial community
upon prolonged propagation will be discussed in the context of PS and CD community dynamics models.
[4]
As indicated previously , the relative abundance of the individual strains belonging to a specific genetic