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O’Connell et al. Microbiome Res Rep 2023;2:21 https://dx.doi.org/10.20517/mrr.2023.17 Page 15 of 21

Figure 7. Novel groups are indicated in bold squares. (A) VIRIDIC-predicted genera of subcluster K1 and K6. The nucleotide similarity
ranges from 46% to 100 %. Based on the genus demarcation of ≥ 70 nucleotide similarity, several novel groups can be identified;
however, a few closely related groups do not have entirely clear distinctions between them; (B) Gegenees analysis of the K1 and K6 MP.
The proteome similarity ranges from 35% to 100 %. This output largely supports the VIRIDIC-predicted genera, but using the
established threshold of ≥ 50% does not completely clarify the boundaries between the proposed genera that appear closely related to
their neighbours. Increasing the threshold to approximately 60% does little to improve this situation; (C) VICTOR-generated
dendrogram of the proposed genera. This phylogenetic tree supports the creation of the novel genera proposed by VIRIDIC based on
their monophyletic nature. Anayavirus was a previously existing genus that has now been reduced in size by the creation of the novel
groups. Therefore, Anayavirus is not a novel finding.