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Page 14 of 21 O’Connell et al. Microbiome Res Rep 2023;2:21 https://dx.doi.org/10.20517/mrr.2023.17
Figure 6. Novel genus assignments within cluster J. Novel groups are indicated within bold squares. (A) VIRIDIC alignment of the
cluster J phages. Nucleotide similarity values range from 52% to 100%. When a similarity threshold of ≥ 60% is applied, two distinct
groups can be observed in the alignment; (B) Gegenees proteome analysis of the cluster J phages. Proteome similarity values range
from 42% to 100%. The two groups observed in the VIRIDIC analysis are reflected in this alignment when a threshold of ≥ 50%
similarity is applied; (C) VICTOR-generated amino acid dendrogram. Two distinct branches are clearly depicted and therefore provide
additional support for the creation of two genera and two subclusters within cluster J.

