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Page 8 of 12 Sane et al. Microbiome Res Rep 2023;2:18 https://dx.doi.org/10.20517/mrr.2023.12
Figure 3. Translocation of bacteria (green) and Lpps (red) to bone marrow cells from MIA-induced (A, B) or sham (C) rats. The two
fluorescent readings (red: TRITC-labeled Lpps or MAE; green: CFSE labeled bacteria) were merged to detect cells capturing Lpps or
MAE (red arrow), bacteria (green arrow) or both bacteria and Lpps (dotted green arrow). TRITC-Labelled Lpps were given at the dose
ranging 0.8-1.1 mg/Kg body weight (A, C). Uptake of the single labelled Lpps was detected (red arrow) in APC+ from the left femur of
OA rat (A) or in APC(-) compartment from the right tibia of sham rat (C). TRITC-labelled MAE was detected either in APC (+) or APC
(-) compartment of femur or tibia (B: APC(-) compartment from the right tibia). Lpps was not detected following administration of the
low dose (140 µg/kg) although bacteria were detected in BM APC(+) from right joint (3.5%-6.6 % total APC(+) and the left one
[8.8%-12.4 % total APC (+)].
bacteria in OA progression is unexpected since both bacteria are considered beneficial, even though
P. goldsteinii can be isolated from infectious samples and Akkermansia is enriched in OA obese patients as
