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Sane et al. Microbiome Res Rep 2023;2:18 Microbiome Research
DOI: 10.20517/mrr.2023.12
Reports

Original Article Open Access

Free lipoproteins from Bifidobacterium longum
alleviate osteoarthritis through modulation of the gut

microbiome

2
1
Famara Sane , Frank Piva , Marie-Bénédicte Romond 2
1
ULR3610, Centre Hospitalier Universitaire de Lille, Lille 59000, France.
2
ULR3610, Université de Lille, Lille 59000, France.
Correspondence to: Dr. Marie-Bénédicte Romond, ULR3610, Université de Lille, Centre Boulanger, rue du Pr. Jules Leclercq, Lille
59000, France. E-mail: marie-benedicte.romond@univ-lille.fr

How to cite this article: Sane F, Piva F, Romond MB. Free lipoproteins from Bifidobacterium longum alleviate osteoarthritis through
modulation of the gut microbiome. Microbiome Res Rep 2023;2:18. https://dx.doi.org/10.20517/mrr.2023.12

Received: 3 Feb 2023 First Decision: 22 Mar 2023 Revised: 29 Mar 2023 Accepted: 15 Apr 2023 Published: 11 May 2023

Academic Editor: Francesca Turroni Copy Editor: Dong-Li Li Production Editor: Dong-Li Li

Abstract
Aim: The “gut-joint” axis is suspected to be involved in the pathophysiology of osteoarthritis (OA). The present
study aims at investigating the potential of lipoproteins (Lpps) secreted by Bifidobacterium longum to alleviate OA
progression in the rat.

Methods: Experimental OA was induced in rats harbouring Schaedler Flora maintained in SPF conditions. Two
weeks post-injection, 20 rats were randomized to water (n = 10) or 0.3 mg/L Lpps solution (n = 10). Weight and
food intake were monitored for 6 weeks. At sacrifice, joints were scored using macroscopic and histological
criteria. Serum LPS, Schaedler flora as well as selected intestinal bacteria were analyzed.

Results: Lpps intake prevents OA progression. The protected rats showed a significant increase in lactobacilli along
the intestine as well as in Mucispirillum schaedleri in the colon and a significant decrease in Parabacteroides goldsteini
and Akkermansia in caecum and colon, respectively. There was no significant difference in serum
lipopolysaccharide or bacteria translocating in Peyer's patches. Labelled Lpps were not detected in bone marrow of
the OA joint. The principal component analysis points out that OA prevention is primarily associated with bacteria
involved in the tryptophane degradation pathway and SCFA formation.

© The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0
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adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as
long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and
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