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activities linked with extreme environmental responses. The first results demonstrated that challenged
oysters in high/medium bathymetry exhibited a low weight gain, increased hexokinase activity, and
increased mitochondrial functioning. We also quantified an up-regulation of AMP-activated protein kinase
(AMPK) activation, a key energy sensor that controls glucose, lipid, and protein metabolism in C. gigas.
Interestingly, up-regulation of AMPK was initially reported as a hallmark of cancer cells to support the
high-energy demand of highly proliferative cells.

Conclusion: We propose the oyster as a new model for cancer research, to identify mechanisms underlying
the ability of cells to adapt to a harsh environment. The oyster is a marine invertebrate that evolved 500
million years ago and we are convinced that it could help us to identify common ancestral pathways for cell
adaptation to a harsh environment, for a better understanding of cancer cells functioning inside the tumor.

47. Resveratrol-induced modulation of non-coding RNA in ovarian cancer cells

1
1
1
1
1
Letizia Vallino , Alessandra Ferraresi , Chiara Vidoni , Claudia Lora , Chinmay Maheshwari ,
Danny N. Dhanasekaran , Ciro Isidoro 1
2
1 Laboratory of Molecular Pathology, Department of Health Sciences, Università del Piemonte Orientale “A.
Avogadro”, Via Solaroli 17, Novara 28100, Italy.
2 Stephenson Cancer Research Center, University of Oklahoma City, Oklahoma City, OK 73104, USA.
Background and aim: Ninety percent of the human genome is transcribed, of which only 2% encodes
[1,2]
proteins; the remaining sequences enclose non-coding genes . Non-coding RNAs (ncRNAs) play a
crucial role in the regulation of several biological processes and their dysregulation may influence cancer
development, functioning as oncogenes or tumor suppressors. ncRNAs generally are divided into two main
groups based on size: long non-coding RNAs (lncRNAs) of about 200 nucleotides, acting as positive or
negative transcription modulators, and microRNAs (miRNAs) of about 20-22 nucleotides, acting as post-
transcriptional silencing molecules . The aim of our work was to investigate whether Resveratrol (RV), a
[3,4]
[5]
polyphenolic compound with anticancer properties , could modulate ncRNAs in ovarian cancer cell lines.
Experimental procedure: Ovarian cancer cells were treated with RV (100 μM). Total RNA was isolated from
the cells and mRNA was amplified and labeled. Labeled specimens were fragmented and hybridized to
Human Whole Genome Oligo Microarrays. One hundred nanograms of total RNA were treated following
the miRNA microarray protocol. RNA was dephosphorylated, denaturated, ligated, and labeled. Samples
were hybridized to Human miRNA Microarray. DIANA TOOLS was used to retrieve predicted microRNA
targets and Gene Ontology for predicting their involvement in biological processes.

Results: We show that RV modulates non-coding transcripts that impact on cancer cell features.

Conclusion: Our data support the view that RV treatment can be effective in cancer therapy on regulating
epigenetic mechanisms involved in cancer development.

REFERENCES
1. Stein LD. Human genome: end of the beginning. Nature 2004;431:915-6.
2. Ponting CP, Belgard TG. Transcribed dark matter: meaning or myth? Hum Mol Genet 2010;19:R162-8.
3. Mendell JT. MicroRNAs: critical regulators of development, cellular physiology and malignancy. Cell Cycle 2005;4:1179-84.
4. Wapinski O, Chang HY. Long noncoding RNAs and human disease. Trends Cell Biol 2011;21:354-61.
5. Elshaer M, Chen Y, Wang XJ, Tang X. Resveratrol: An overview of its anti-cancer mechanisms. Life Sci 2018;207:340-9.