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Page 28 of 38 J Cancer Metastasis Treat 2020;6:5 I http://dx.doi.org/10.20517/2394-4722.2020.13

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39. DNA methylation markers for noninvasive detection of early stage colorectal cancer

1
1
1
Yanqun Liu , Min Hoe Chew , Wah Siew Tan , Choong Leong Tang , Yi Zhao 2
1
1 Department of Colorectal Surgery, Singapore General Hospital, Singapore 169608, Singapore.
2 Department of clinical Research, Singapore General Hospital, Singapore 169608, Singapore.
Background and aim: Colorectal cancer (CRC) is the most common cancer in Singapore. Earlier detection
enhances chances of a cure and facilitates reducing CRC mortality rates. Colonoscopy is currently the gold
standard for CRC diagnosis, but a somewhat troublesome and invasive procedure makes its acceptance
not high in the general public as a screening tool. Epigenetic silencing of tumor-related genes by promoter
methylation is common in CRC, but no biomarker has been proven to be individually of sufficient
sensitivity or specificity in routine clinical practice. The aim of this study was to identify tumor-derived
methylated genes in the serum of stage IIA CRC and assess their diagnostic potentials for early stage of
colorectal cancer.

Experimental procedure: In this prospective study, DNA methylation levels were measured by quantitative
methylation-specific PCR. Two genes (PPP1R3C and ADHFE1) were first investigated in serum samples of
an exploratory set of stage IIA CRC case-controls. Methylation results were verified in the sera of a test set
compromising 50 stage IIA cases and 50 age- and gender-matched healthy controls. The receiver operating
characteristic curve was constructed for assessment of assay performance.

Results: Serum methylation levels of PPP1R3C and ADHFE1 were significantly higher in stage IIA patients
as compared to healthy controls (both P < 0.001, Mann-Whitney U test). Areas under the receiver operating
curve (AUCs) using serum methylation levels of PPP1R3C and ADHFE1 were 0.60 [95% confidence interval
(CI), 0.48-0.71] and 0.73 (95%CI, 0.62-0.83), respectively. At a specificity of 80%, the assay sensitivities of
methylated PPP1R3C and ADHFE1 were 26% and 56%, respectively.

Conclusion: Serum methylation levels of ADHFE1 might be useful for minimally invasive detection of
early stage II colorectal cancer. Validation studies in larger and independent cohorts and identification of
additional markers are necessary.

40. Quantification of HER2 protein using multiple reaction monitoring-mass spectrometry in

formalin-fixed paraffin-embedded (FFPE) breast cancer tissue specimens

Misol Do , Hyunsoo Kim , Han Suk Ryu , Youngsoo Kim 1,2
2
3
1
1 Departments of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Korea.
2 Departments of Biomedical Engineering, Seoul National University College of Medicine, Seoul 03080, Korea.
3 Departments of Pathology, Seoul National University College of Medicine, Seoul 03080, Korea.

Human epidermal growth factor receptor 2 (HER2) protein is often overexpressed in breast cancer and
is correlated with a worse prognosis; thus, accurate detection of HER2 by using optimum techniques is