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Page 6 of 11 Tran et al. J Cancer Metastasis Treat 2020;6:47 I http://dx.doi.org/10.20517/2394-4722.2020.104

Figure 1. ROR2 regulated lung metastasis of LM8. A: gene set enrichment analysis. Microarray data for LM8-H and LM8-L were analyzed
using Kyoto Encyclopedia of Genes and Genomes software to identify significant gene sets. The enrichment plot shows the distribution
of genes in the set that are correlated with the Wnt signaling pathway (mm0431); B: mRNA expression levels of Ror2, Ror1, and Fzd1 in
LM8-H and LM8-L analyzed by RT-PCR. The numbers below the bands indicate the corresponding expression levels relative to β-actin; C:
ROR2 protein expression levels in LM8-L, LM8-H, and H/Ror2-KO examined by western blotting; D: proliferation rates of LM8-H, LM8-L,
and H/Ror2-KO cultured under adhesion conditions for 3 days; E: representative lung images stained with hematoxylin-eosin (left) and
the number of lung foci bigger than 1 mm in diameter (right) at day 20 after intravenous injection of LM8-H and H/Ror2-KO. Scale bars:
10 mm, n = 5, **P < 0.01. ROR2: receptor tyrosine kinase-like orphan receptor 2; LM8-H: LM8 cell line with high metastatic ability; LM8-L:
LM8 cell line with low metastatic ability; Fzd1: Frizzled class receptor 1; H/Ror2-KO: LM8-H knocked out of Ror2; n.s: not significant