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Table 5. Spearman correlation analysis of 11 mitochondrial genome mutations with cardiac angina
Mutation Correlation coefficient Significance
m.12315G>A 0.079 0.121
m.652delG 0.061 0.224
m.3336T>C 0.047 0.342
m.14459G>A 0.116 0.034**
m.5178C>A 0.092 0.068*
m.13513G>A -0.075 0.129
m.652insG -0.078 0.124
m.3256C>T 0.069 0.218
m.15059G>A -0.122 0.036**
m.1555A>G -0.065 0.217
m.14846G>A -0.068 0.222

**P ≤ 0.05; *P ≤ 0.1

MtDNA mutations m.14459G>A and m.5178C>A can be used for evaluation the predisposition of individuals
to atherosclerotic lesions. At the same time, mitochondrial genome mutation m.15059G>A may be used for
gene therapy of atherosclerosis.

DECLARATIONS
Authors’ contributions
Conception, design and statistical analysis: Sazonova MA
Pyrosequencing of PCR fragments: Sazonova MA, Sinyov VV
PCR: Ryzhkova AI, Shkurat TP
DNA extraction: Sazonova MD, Nikitina NN
Administrative and material support: Sobenin IA, Orekhov AN

Availability of data and materials
The data were strictly obtained from medical records according to the privacy policy and ethics code of our
institute.

Financial support and sponsorship
This work was supported by the Russian Foundation for Basic Research (19-015-00479).

Conflicts of interest
All authors declared that there are no conflicts of interest.

Ethical approval and consent to participate
The study was carried out in accordance with the Declaration of Helsinki. The study protocol was inspected
and approved by the Ethics Committee of the Institute of General Pathology and Pathophysiology. Each
study participant has signed a written informed consent to participate in this investigation.

Consent for publication
Not applicable.

Copyright
© The Author(s) 2019.

REFERENCES
1. Hoffmann U, Massaro JM, D’Agostino RB Sr, Kathiresan S, Fox CS, et al. Cardiovascular event prediction and risk reclassification by
coronary, aortic, and valvular calcification in the Framingham heart study. J Am Heart Assoc 2016;5:e003144.