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El-Ghoneimi et al. Plast Aesthet Res 2022;9:39  https://dx.doi.org/10.20517/2347-9264.2021.101  Page 3 of 11

               Table 1. Patient data and results
                                Number of        Lenght of
                Case Indication  previous   Age   urethroplasty   Fu   Complications  Treatment of complications
                                                             (months)
                                          (years)
                                surgeries        (cm)
                1   Multiple operated  7  11.3   12          97      None
                    perineal
                    hypospadias
                2               12        12.9   15          62      Meatal stenosis, stone  Stone laser fragmentation, self-
                                                                                     dilatation 3 years, no recurrence
                3   Ovotesticular   1     12.5   14          61      Proximal fistula  Fistula closure, no further
                    46XX DSD                                                         surgery
                4               1         10.5   12          106     Proximal fistula  Fistula closure, no further
                                                                                     surgery
                5   Perineal    0         1.7    8           160     Proximal stenosis  Onlay buccal mucosal graft, no
                    hypospadias                                                      further surgery
                    staged repair
                6               0         7.1    9           41      none
                7               0         2      7           30      stricture       Total redo, staged buccal
                                                                                     mucosal graft complicated by
                                                                                     proximal fistula needed closure
                                                                                     without recurrence
                8               0         6.6    8           6       Lost of FU (follow-up)
                                                                     (after 6 months free of
                                                                     complications)
                9   Urethral    2         1.7    8           71      none
                    duplication
                10  Circumcision   2      16.3   16          18      none            Preventive self-dilatation 12
                    complication                                                     months

               DSD: Disorder of sex development.


               cases had severe curvature > 45°; treatment was done by removal of all fibrous tissues and ventral
               corporoplasty when needed. (A ventral transverse incision was made at the point of greatest curvature
               through the tunica albuginea, extending from the 3 o’clock to the 9 o’clock position.) A flap of tunica
               vaginalis was used to cover the corpora defect. In cases of unavailable tunica vaginalis (ovotesticular DSD),
               multiple fairy cuts were done. All reconstructed or fibrous urethra was removed until obtaining a healthy
               native urethra; all of them were perineal [Figures 1 and 2].

               In redo cases, penile skin was reconstructed either by local flaps or by free skin grafts. A minimum delay of
               one year was respected before TBMG.


               Bladder mucosa graft harvesting by minimal detrusotomy
               A short suprapubic transversal skin incision was done. The bladder was filled with saline and accessed by
               midline facial incision. A short (2 cm in length) transversal detrusotomy was done on the anterior wall of
               the bladder [Figure 3]. The exposed mucosa was incised vertically and retracted by 5/0 stich. Two parallel
               perpendicular incisions were done to isolate 2 cm width of the mucosa. Blunt and sharp dissection was done
               at the submucosal plane with mild retraction and progressive incision of the two parallel lines. These
               incisions were limited to the needed length for the urethra [Supplementary Video 1]. The mucosal defect
               was sutured by a running 5/0 absorbable suture, and the detrusotomy was closed after insertion of a supra
               pubic catheter.


               Tubularization of the graft
               The graft was tubularized by interrupted 6/0 absorbable polydioxanone (PDS, Ethicon) round needle. The
               tabularization was done around a Foley catheter of at least Ch 12 according to the age of the child. The graft
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