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Page 10 of 13 Huang et al. Microbiome Res Rep 2024;3:30 https://dx.doi.org/10.20517/mrr.2024.14
Figure 4. F. prausnitzii has a higher impact than the microenvironment on the expression of transcription factor genes in the colonic
epithelial cells. (A)Volcano plot on the gene expression of transcription factors in GuMI-FP vs. GuMI-NB. The 260 significantly changed
genes (P < 0.05, log2(|FC|) > 1) are highlighted in red; and (B) volcano plot on the gene expression of transcription factors in GuMI-NB
vs. GuMI-FP. The 27 significantly changed genes (P < 0.05, log2(|FC|) > 1) are highlighted in red; (C) Venn plot on the significantly
changed transcription factor indicates the unique effects of F. prausnitzii. Seventeen transcription factors are changed by
microenvironment change (NB vs. Static) and F. prausnitzii (FP vs. NB). GuMI-FP: GuMI with F. prausnitzii; GuMI-NB: GuMI without
bacteria.
Figure 5. Overview of using GuMI microphysiological system and metabolomics to identify new metabolic activities of F. prausnitzii in
modulating chemical profile in the intestinal lumen and inducing a transcriptional change in colonic epithelial cells.
