Page 23 - Read Online

Huang et al. Microbiome Res Rep 2024;3:30 Microbiome Research
DOI: 10.20517/mrr.2024.14
Reports

Short Report Open Access

Faecalibacterium prausnitzii A2-165 metabolizes
host- and media-derived chemicals and induces

transcriptional changes in colonic epithelium in
GuMI human gut microphysiological system

1
1
2,3
1
1
Yu-Ja Huang , Caroline A. Lewis , Charles Wright , Kirsten Schneider , John Kemmitt , David L.
4
1
5
Trumper , David T. Breault , Omer Yilmaz , Linda G. Griffith 1,4,6 , Jianbo Zhang 1,7,8
1
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
2
Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
3
current address: UMass Chan Medical School, Program in Molecular Medicine, Worcester, MA 01605, USA.
4
Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
5
Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA.
6
Center for Gynepathology Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
7
Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam 1098 XH, the Netherlands.
8
Tytgat Institute for Liver and Intestinal Research, Amsterdam Gastroenterology, Endocrinology and Metabolism, Amsterdam
UMC, Location Academic Medical Center, Amsterdam 1105 BK, the Netherlands.
Correspondence to: Dr. Jianbo Zhang, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904,
Amsterdam 1098 XH, the Netherlands. E-mail: j.zhang6@uva.nl
How to cite this article: Huang YJ, Lewis CA, Wright C, Schneider K, Kemmitt J, Trumper DL, Breault DT, Yilmaz O, Griffith LG,
Zhang J. Faecalibacterium prausnitzii A2-165 metabolizes host- and media-derived chemicals and induces transcriptional changes
in colonic epithelium in GuMI human gut microphysiological system. Microbiome Res Rep 2024;3:30. https://dx.doi.org/10.
20517/mrr.2024.14
Received: 27 Feb 2024 First Decision: 8 Apr 2024 Revised: 18 Apr 2024 Accepted: 8 May 2024 Published: 22 May 2024
Academic Editors: Marco Ventura, Xu Zhang Copy Editor: Dong-Li Li Production Editor: Dong-Li Li
Abstract
Aim: Recently, a GuMI gut microphysiological system has been established to coculture oxygen-intolerant
Faecalibacterium prausnitzii (F. prausnitzii) A2-165 with organoids-derived primary human colonic epithelium. This
study aims to test if this GuMI system applies to different donors with different healthy states and uses
metabolomics to reveal the role of gut microbes in modulating host- and diet-derived molecules in the gut lumen.
Methods: Organoids-derived colonic monolayers were generated from an uninflamed region of diverticulitis,
ulcerative colitis, and Crohn’s disease patients and then integrated into the GuMI system to coculture with
F. prausnitzii A2-165 for 2 to 4 days. Apical media was collected for metabolomic analysis. Targeted metabolomics

© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0
International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, sharing,
adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as
long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and
indicate if changes were made.

www.oaepublish.com/mrr