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Kok et al. Microbiome Res Rep 2023;2:30 https://dx.doi.org/10.20517/mrr.2023.16 Page 13 of 19

Figure 6. In vitro testing of phage cocktails. (A) Four cocktails were tested for their effectiveness against each of four bacterial strains:
PFR-Pl-2017, PFR-Pl-2006, W19-08100, and Pl-P1627. Strain Pl-P1627 was known to be resistant to all phages in this study; (B) Tables
to summarize the results of cocktail testing and the substitution of Phage Callan for Phage Dash in Cocktails 2 and 4. Table colours
represent the host range observations. Black: plaques; grey: full clearings; white: no infection. The symbols within the table record the
overall effectiveness of the cocktail that phage is in on that host strain: +: effective cocktail; -/+: partially effective cocktail; -: ineffective
cocktail.

To better understand how these isolates might interact with phages, we screened these preliminary genomes
for positive signs of phage defense mechanisms. We found each of our isolates contained CRISPR arrays.