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Mezhyrova et al. Microbiome Res Rep 2023;2:28 https://dx.doi.org/10.20517/mrr.2023.28 Page 11 of 17
Figure 4. Cluster formation of full-length MS2-L derivatives in E. coli Lemo21 (DE3) bacterial cells. Toxin expression was induced at
OD = 0.3 with 1 mM IPTG and samples were taken at defined time points after induction. All images were acquired with an LSM700
600
confocal microscope with a 63× objective (scale bar = 5 µm). (A) Cells synthesizing MS2-L-GFP 45 min after induction; (B) Cells
synthesizing mScarlet-MS2-L 40 min after induction; (C) Propidium iodide staining of cells synthesizing MS2-L-GFP 45 min after
induction and of Lemo21 (DE3) control cells. The vast majority of the control cells remain unstained; (D) Co-expression of MS2-L-GFP
and mScarlet-MS2-L, 20 and 40 min after induction. GFP: Green fluorescent protein; IPTG: isopropyl-β-D-thiogalactopyranoside.
