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Page 10 of 17 Mezhyrova et al. Microbiome Res Rep 2023;2:28 https://dx.doi.org/10.20517/mrr.2023.28
Figure 3. Oligomeric assembly of MS2-L derivatives in NDs and in detergent environments. MS2-L derivatives were CF expressed in the
presence of either 60 µM DMPG NDs (black traces) or 0.4% Brij78 (red traces), purified and subjected to LILBID analysis. Samples
were synthesized in the standard A19 lysate (left panel) or in BW25113ΔDnaJ lysate (right panel). Pictograms illustrate complexes
assigned to the respective peak. For reasons of clarity, not each peak has been assigned a pictogram. Laser intensities were adjusted
between 10 and 20 mJ to optimize ion yield while preserving high-order complexes. Asterisks mark prominent peaks representing
impurities. The individual MS2-L derivatives are indicated. DMPG: 1,2-dimyristoyl-sn-glycero-3-phospho-(1’-rac-glycerol); LILBID:
laser-induced liquid bead ion desorption; ND: nanodisc.
