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Page 4 of 14 Millen et al. Microbiome Res Rep 2023;2:26 https://dx.doi.org/10.20517/mrr.2023.29

DGCC6071 Starter strain 6073-like EPS EpsM var. This study
DGCC6073 Starter strain 6073-like EPS typical [18]
DGCC6871 Starter strain 6073-like EPS EpsM var. This study
DGCC7168 Starter strain 6073-like EPS EpsM var. This study
DGCC7193 Starter strain 6073-like EPS EpsM var. This study
DGCC8692 Starter strain 6073-like EPS typical This study
LM2345 Plasmid-free, Sp R [20]
R +
2345-EPS Sp , pEPS6073 [18]
2345-Dit 6887 LM2345 + pG9Dit 6887 This study
Escherichia coli
+
q
TG1 RepA + SupE hsd 5 thi (kac-proAB) F’ (traD36 proAB lacI lacZ M15) with chromosomal copy of [21]
pWV01 repA
Bacteriophages
D753 Host DGCC6871 This study
D970 Host DGCC8692 This study
D1113 Host DGCC8692 This study
D2929 Host DGCC7168 This study
D4006 Host DGCC7193 This study
D4839 Host DGCC6073 This study
D4842 Host DGCC6071 This study
D5604 Host DGCC6071 This study
D6067 Host DGCC8692 This study
D6869 Host DGCC6073 This study
D6875 Host DGCC6073 This study
D6887 Host DGCC6073 This study
D6888 Host DGCC6073 This study
p2 Host LM2345 [22]
bIL170 Host 1403S [23]
[24]
P008NC Host 1403S; derivative of P008 North Carolina State
collection
p2-Dit p2 encoding Dit This study
6887 6887
bIL170-Dit 6887 bIL170 encoding Dit 6887 This study
P008NC-Dit P008NC encoding Dit This study
6887 6887
Plasmids
R
pGhost9 Em , temperature sensitive vector [25]
pG9Dit 6887 pGhost9 + Dit 6887 This study
R R R
Dit: Distal tail protein; Em : erythromycin-resistant; EPS: exopolysaccharide; Sm : streptomycin-resistant; Sp : spectinomycin-resistant; var.:
variant.

PCR, DNA preparation, sequencing, and bioinformatic tools
PCRs were performed with GoTaq® Colorless Master Mix (Promega Corp., USA) or Phusion HF Mastermix
(Thermo Scientific, USA) according to the manufacturer’s instructions with 35 cycles of denaturation/
annealing/extension. Primer sequences and additional thermocycler conditions are listed in Table 2.
Amplicons were purified using the Promega Wizard® SV Gel and PCR Clean-Up System (Promega Corp.,
USA). Primers were synthesized by Integrated DNA Technologies (USA). Phage DNA was isolated from
high-titer phage lysate using Invitrogen PureLink Viral RNA/DNA Mini kit (Life Technologies, USA)
according to the manufacturer’s instructions. Phage DNA was sequenced using Illumina technology
(Illumina, USA). Sanger sequencing was performed by Eurofins Genomics (USA). Sequences were