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Page 8 of 12 Kikukawa et al. Microbiome Res Rep 2023;2:4 https://dx.doi.org/10.20517/mrr.2022.24

Table 2. Fatty acid compositions of TFA samples obtained from the JCM 7042 strain cultured in TOS or TOS + 5.0 g/L Tween 80
TFA Fatty acid ratio (%) in TFA
(mg/L) C12:0 C14:0 C16:0 7-cis-C16:1 9-cis-C16:1 C18:0 9-cis-C18:1 Others a
TOS 23.1 4.5 21.9 26.7 6.1 0.3 1.5 18.7 20.3
TOS + 5.0 g/L Tween 80 12.0 0.2 0.7 39.2 0.1 0 2.3 39.5 18.1
a
Others include some fatty acids with small ratios in the TFA, such as C10:0, C17:0, trans-C18:1, cycloC19, etc; TFA: total fatty acids.

Figure 4. Effect of medium components on the 7-cis-C16:1 content in the JCM 7042 cells. (A) Each of TOS and MRS medium
components was added to the MRS and TOS media, respectively, at the concentration of 3.0 g/L for (NH ) SO ; 0.5 g/L for L-cysteine;
4 2
4
10 g/L for galactooligosaccharide (GOS); 3.0 g/L for K HPO , 1.0 g/L for Tween 80, 2.0 g/L for ammonium citrate (Citrate); 10 g/L for
2
4
beef extract; 5.0 g/L for sodium acetate (Acetate); and 20 g/L for glucose; (B) effect of Tween 80 on the 7-cis-C16:1 content and post-
culture cell biomass of the JCM 7042 strain.

Figure 5. Antibacterial activity of TFA extracted from the JCM 7042 strain against S. aureus (A) and S. epidermidis (B) strains. The TFA
were used as the form of acid-hydrolyzed FFAs from total lipids. FFA: Free fatty acid; TFA: total fatty acids.

DISCUSSION
In this study, we revealed that Bifidobacterium sp. JCM 7042 produces 7-cis-C16:1. The fatty acid was not
detected or is produced under the detection limit for other species, such as B. bifidum, B. catenulatum, B.
indicum, B. longum, and B. pseudocatenulatum. Moreover, C16:1-producing ability varied even within the
same species, suggesting that there are differences in genetic and/or enzymatic contexts involved in fatty