Page 37 - Read Online

Page 12 of 38 J Cancer Metastasis Treat 2020;6:5 I http://dx.doi.org/10.20517/2394-4722.2020.13

16. Sensitive detection of metabolic abnormalities in adult T-cell leukemia/lymphoma and
induction of specific leukemic cell death using photodynamic therapy

4
1
1,2
Takashi Oka , Hajime Mizuno , Masumi Sakata , Hirofumi Fujita , Tadashi Yoshino , Yoshihisa
3,7
3
3
4
Yamano , Kozo Utsumi , Tsutomu Masujima , Atae Utsunomiya 6
5
1 Department of Pathology and Oncology, Graduate School of Medicine, Dentistry and Pharmaceutical
Sciences, Okayama University, Okayama 700-8558, Japan.
2 Department of Hematology, Oncology and Respiratory Medicine, Graduate School of Medicine, Dentistry and
Pharmaceutical Sciences, Okayama University, Okayama 700-8558, Japan.
3 Laboratory for Single Cell Mass Spectrometry, RIKEN Quantitative Biology Center (QBiC), Osaka 565-0874,
Japan.
4 Department of Cytology and Histology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,
Okayama University, Okayama 700-8558, Japan.
5 Department of Rare Diseases Research, Institute of Medical Science, St. Marianna University School of
Medicine, Kawasaki 216-8512, Japan.
6 Department of Hematology, Imamura General Hospital, Kagoshima 890-0064, Japan
7 Present address: Laboratory of Analytical and Bioanalytical Chemistry, School of Pharmaceutical Sciences,
University of Shizuoka, Shizuoka 422-8526, Japan.

Adult T-cell leukemia/lymphoma (ATL) is an aggressive T-cell neoplasm caused by human T-cell leukemia
virus type I (HTLV-I). Therapeutic interventions have not been associated with satisfactory outcomes. We
showed that the porphyrin metabolic pathway preferentially accumulates the endogenous photosensitive
metabolite, protoporphyrin IX (PpIX), in ATL, after a short-term culture with 5-aminolevulinic acid (ALA).
PpIX accumulated 10-100-fold more in ATL leukemic cells when compared to healthy peripheral blood
mononuclear cells (PBMCs). Patient specimens showed dynamic changes in flow cytometry profiles during
the onset and progression of ATL. Furthermore, 98.7% of ATL leukemic cell death in the ATL patient
specimens could be induced with 10 min of visible light exposure, while 77.5% of normal PBMCs survived.
Metabolomics analyses revealed that a specific stage of the metabolic pathway progressively deteriorated
with HTLV-I infection and at the onset of ATL. Therefore, this method will be useful for diagnosing and
identifying high-risk HTLV-I carriers and high-risk indolent ATL patients who appeared to have developed
or are likely to develop the aggressive subtypes with single cell resolution. Photodynamic therapy in the
circulatory system may be a potential treatment due to its highly-specific, non-invasive, safe, simultaneous,
and repeatedly-treatable modalities.

17. Preoperative localization of breast lesions: analysis of current techniques

Ray Cody Mayo

The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

Image-guided preoperative localization of breast lesions is a relatively common procedure. This
presentation describes the most common localization options available commercially - wire localization,
radioactive seed localization, radiofrequency reflector localization, and magnetic seed localization - and
outlines the advantages and disadvantages of each. This information may help radiologists, surgeons,
pathologists, and hospital administration as they seek to add value and provide patient-centered care.