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J Cancer Metastasis Treat 2020;6:5 I http://dx.doi.org/10.20517/2394-4722.2020.13 Page 11 of 38

Results: Hydrodynamic diameter (d ) of DMTre composed of 30 mol% DMPC and 70 mol% TreC14 was
hy
100 nm with single and narrow range of size distribution, which can avoid reticular endothelial system
in vivo. An increase in TFAL values of DMTre was obtained in a dose-dependent manner. DMTre inhibited
the growth of breast and lung tumor cells leading to apoptosis with the activation of caspases. The
suppression of tumor weight of xenograft mice model of carcinoma treated with DMTre after inoculation
with breast tumor cells was obtained along with apoptosis. A remarkable reduction of volume and weight
in subcutaneous tumors on subcutaneous lung carcinoma-bearing mice administered with DMTre was
obtained.

Conclusion: Anti-tumor activities of DMTre against carcinoma-bearing mice along with apoptosis were
obtained. The results of this study could contribute to the development of therapeutic agents for patients
with carcinoma in future clinical application.

REFERENCES
1. Matsumoto Y, Cao E, Ueoka R. Growth inhibition by novel liposomes including trehalose surfactant against hepatocarcinoma cells along
with apoptosis. Anticancer Res 2013;33:4727-40.
2. Matsumoto Y, Kuwabara K, Ichihara H, Kuwano M. Therapeutic effects of trehalose liposomes against lymphoblastic leukemia leading to
apoptosis in vitro and in vivo. Bioorg Med Chem Lett 2016;26:301-5.

15. The effect of interactions between temozolomide and dexamethasone on the profile of 84

selected proteins in glioblastoma multiforme cells

Anna Bielecka-Wajdman, Tomasz Ludyga, Ewa Obuchowicz

Department of Pharmacology, School of Medicine in Katowice, Medical University of Silesia, Katowice 40-055,
Poland.

Background and aim: In patients with glioblastoma multiforme (GBM), standard chemotherapy with
temozolomide (TMZ) is always supplemented with dexamethasone (DXM). Even though for years DXM
has been applied as a “gold standard” in therapy of vasogenic edema, intracranial pressure, and mass effect,
recent controversial results have challenged the widely accepted dogma concerning its using in therapy of
GBM. The results of experimental studies emphasize that DXM may increase the aggressiveness of GBM by
promoting the proliferation and invasiveness of cancer cells.

The aim of our study conducted on two primary glioblastoma lines obtained from patients and on the
commercial line T98G was to assess the effects of TMZ and DXM, as well as their interaction, on the
profile of 84 proteins involved in the process of carcinogenesis. The tests were performed using the
Proteome Profiler Human XL Oncology Array Kit (R & D) in cells cultured under two oxygen conditions:
physiological for tumor hypoxia (2.5% oxygen) or in standard laboratory conditions (20% oxygen)
frequently used in in vitro studies.

Results: Our results confirm the pro-tumorigenic properties of DXM but they also show that the response
of GBM commercial and primary cell lines to DXM given to culture medium with or without TMZ is
variable and depends on oxidation of the microenvironment.

Conclusion: It can be concluded that DXM and TMZ administered together or separately may induce
different effects which depend on the degree of hypoxia prevailing in the malignant brain tumor.