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Morgan et al. J Cancer Metastasis Treat 2018;4:46 Journal of Cancer
DOI: 10.20517/2394-4722.2018.19 Metastasis and Treatment

Original Article Open Access

Increased ARF6 activation correlates with HGF
stimulation in non-invasive prostate cancer cells

Claire Morgan, Lucy J. Swithenbank, Helen Whiteland, Shareen H. Doak

Swansea University Medical School, Institute of Life Science, Swansea University, Swansea SA2 8PP, UK.

Correspondence to: Dr. Claire Morgan, Swansea University Medical School, Institute of Life Science, Swansea University,
Swansea SA2 8PP, UK. E-mail: C.Morgan@swansea.ac.uk

How to cite this article: Morgan C, Swithenbank LJ, Whiteland H, Doak SH. Increased ARF6 activation correlates with HGF
stimulation in non-invasive prostate cancer cells. J Cancer Metastasis Treat 2018;4:46.
http://dx.doi.org/10.20517/2394-4722.2018.19

Received: 7 Mar 2018 First Decision: 11 May 2018 Revised: 30 Jul 2018 Accepted: 31 Jul 2018 Published: 30 Aug 2018

Science Editors: Klaus Pors Copy Editor: Yuan-Li Wang Production Editor: Huan-Liang Wu

Abstract
Aim: The effects of hepatocyte growth factor (HGF) on a non-invasive prostate cancer cell line (CAHPV-10), expressing
cMET were studied, to mimic the possible effects neo-adjuvant androgen deprivation therapy may have in promoting
tumour progression.

Methods: Prostate epithelial cells and prostate cancer cells derived from cancer metastatic sites were analysed using
cell culture assays, immunofluorescence, quantitative real-time polymerase chain reaction and western blotting, with or
without HGF stimulation.

Results: HGF significantly enhanced cell proliferation and induced cell scattering and invasion in CAHPV-10 cells
compared to untreated controls. Active adenosine diphosphate-ribosylation factor 6 (ARF6) was found to be present in
all metastatic prostate cancer cells, with levels highest in the most aggressive cell line, PC-3. Following stimulation with
HGF, active ARF6 expression was substantially elevated in CAHPV-10 cells.

Conclusion: These findings provide further molecular insight into the progression of prostate cancer and highlights
potential issues for early prostate cancer therapeutic strategies.

Keywords: Prostate cancer, adenosine diphosphate-ribosylation factor 6, hepatocyte growth factor stimulation, androgen
deprivation therapy

© The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0
International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use,
sharing, adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as long
as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license,
and indicate if changes were made.

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