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J Cancer Metastasis Treat 2016;2 Suppl 1:A1-30 Journal of
DOI: 10.20517/2394-4722.2016.58
Cancer Metastasis and Treatment

www.jcmtjournal.com
Meeting Abstracts Open Access

Obergurgl Research Conference Abstracts

on "Cancer Stem Cells: Impact on

Treatment"

Tyrol, Austria; 7-11 December 2016; Published: 16 November 2016

Correspondence to: Prof. Dr. Ira-Ida Skvortsova, Laboratory for Experimental and Translational Research on Radiation Oncology (EXTRO-Lab),
Department of Therapeutic Radiology and Oncology, Innsbruck Medical University, Anichstr. 35, A-6020 Innsbruck, Austria.
E-mail: Ira.Skvortsova@i-med.ac.at; Ira.Skvortsova@tirol-kliniken.at

A1 Akt/mTOR pathway is closely linked with EMT and
Proteomics identification of protein CSCs expression. Therefore, these CaP-RR cells,
representative of the source of recurrence after
biomarkers and signaling pathways for RT, provide a very good model to mimic the clinical
prostate cancer radioresistance therapy radioresistance condition to find biomarkers and
signaling pathways for CaP radiotherapy.
1,2
Lei Chang , Peter Graham , Jingli Hao , Valerie
1,2
1,2
3,4
Wasinger , Jie Ni , Julia Beretov 1,2,5 , Junli Deng , Aim: The objective of this study was to identify
1,2
1,2
Joseph Bucci , David Malouf , David Gillatt , Yong Li 1,2 candidate proteins and the main signaling pathways
1,2
6
6,7
involved in CaP radioresistance, validate the identified
1 Cancer Care Centre, St. George Hospital, Kogarah, Australia;
2 St. George and Sutherland Clinical School, Faculty of Medicine, UNSW, potential biomarkers in CaP-radioresistant (RR)
Kensington, Australia; cell lines and animal xenografts, and perform the
3 Bioanalytical Mass Spectrometry Facility, Mark Wainwright Analytical functional study from a selected candidate. Methods:
Centre, UNSW, Kensington, Australia;
4 School of Medical Science, UNSW, Kensington, Australia; The differential proteins from CaP parental cell lines
5 SEALS, Anatomical Pathology, St. George Hospital, Kogarah, Australia; (PC-3, DU145 and LNCaP) and CaP-RR sublines
6 Department of Urology, St. George Hospital, Kogarah, Australia;
7 Australian School of Advanced Medicine, Macquarie University, Sydney, (PC-3RR, DU145RR and LNCaPRR) were analyzed
Australia using LC-MS/MS and identified by a label-free ion
count approach. Pathways enriched as a result of
Background: Radioresistance is a major problem radioresistance were assessed. Identified potential
in prostate cancer (CaP) radiotherapy (RT). The markers were validated in CaP-RR cell lines and
mechanisms of CaP radioresistance are still unclear. subcutaneous (s.c) animal xenografts by Western
We have recently developed CaP-RR (radioresistant) blotting and immunohistochemistry. In addition, the
cell lines which display more aggressive characteristics protein fructose-bisphosphate aldolase A (ALDOA)
including increased colony formation, invasion ability, was identified as a key protein in radioresistance and
sphere formation capability, and enhanced epithelial was selected for radiosensitivity study. Results: A total
mesenchymal transition (EMT) and cancer stem cell of 309 signaling pathway proteins were identified to be
(CSC) phenotypes. In addition, we found the PI3K/ significantly different between CaP and CaP-RR cells
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