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Vander Borght et al. J Cancer Metastasis Treat 2019;5:57 I http://dx.doi.org/10.20517/2394-4722.2019.0010 Page 5 of 6

Figure 4. Strategy for the isolation of extracellular vesicles (EVs) derived from SCLC cells. SCLC-specific EVs are enriched and purified
by RNL-1 and/or 123C antibodies, specific for the extracellular moiety of NCAM [19-21] , coupled to magnetic beads. In the next step the
presence of E18 in the EVs will be measured with one of the specific antibodies. We will choose the antibody with the best signal to noise
ratio

plasma membrane. The membranes of EVs contain in principle all cellular surface proteins and, within the
membrane, all cytoplasmic components . Virtually all SCLC cells express NCAM [16,17] . We will enrich for
[15]
NCAM-containing EVs, using immunoaffinity capture methods [Figure 4].
[18]

DECLARATIONS
Authors’ contributions
Design and execution of experiments: Vander Borght A, Duysinx M, Ummelen M
Manuscript drafting, literature analysis and research strategy: van der Zeijst BAM

Availability of data and materials
Not applicable.

Financial support and sponsorship
None.

Conflicts of interest
All authors declared that there are no conflicts of interest.

Ethical approval and consent to participate
The animal experiments were carried out at the University of Maastricht, and were approved by the Animal
Experiments Committee of the University of Maastricht (project number 2005-043).

Consent for publication
Not applicable.

Copyright
© The Author(s) 2019.

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