Page 105 - Read Online
P. 105

Page 8 of 9                                  Lai et al. J Cancer Metastasis Treat 2019;5:65  I  http://dx.doi.org/10.20517/2394-4722.2019.011

               DECLARATIONS
               Authors’ contributions
               Made substantial contributions to conception: Lai RC, Lim SK
               Provided initial drafts of the work: Lai RC
               Revising the work critically for important intellectual content: Tan KH, Lim SK
               Final approval of the version: Lim SK

               Availability of data and materials
               Not applicable.

               Financial support and sponsorship
               None.

               Conflicts of interest
               Lim SK holds founder shares in Paracrine Therapeutics Pte Ltd.

               Ethical approval and consent to participate
               Not applicable.

               Consent for publication
               Not applicable.

               Copyright
               © The Author(s) 2019.


               REFERENCES
               1.   Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and
                   mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 2018;68:394-424.
               2.   Lötvall J, Hill AF, Hochberg F, Buzás EI, Di Vizio D, et al. Minimal experimental requirements for definition of extracellular vesicles and
                   their functions: a position statement from the International Society for Extracellular Vesicles. J Extracell Vesicles 2014;3:26913.
               3.   Thery C, Witwer KW, Aikawa E, Alcaraz MJ, Anderson JD, et al. Minimal information for studies of extracellular vesicles 2018
                   (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J
                   Extracell Vesicles 2018;7:1535750.
               4.   Lane RE, Korbie D, Hill MM, Trau M. Extracellular vesicles as circulating cancer biomarkers: opportunities and challenges. Clin Transl
                   Med 2018;7:14.
               5.   Zhang P, Samuel G, Crow J, Godwin A, Zeng Y. Molecular Assessment of Circulating Exosomes towards Liquid Biopsy Diagnosis of
                   Ewing Sarcoma Family of Tumors. Transl Res 2018;201:136-153.
               6.   Schwich E, Rebmann V. The Inner and Outer Qualities of Extracellular Vesicles for Translational Purposes in Breast Cancer. Front Immunol
                   2018;9:584.
               7.   Fujita Y, Kuwano K, Ochiya T, Takeshita F. The Impact of Extracellular Vesicle-Encapsulated Circulating MicroRNAs in lung cancer
                   research. Biomed Res Int 2014;2014:486413.
               8.   Verma M, Lam TK, Hebert E, Divi RL. Extracellular vesicles: potential applications in cancer diagnosis, prognosis, and epidemiology.
                   BMC Clin Pathol 2015;15:6.
               9.   Anderson NL, Anderson NG. The human plasma proteome: history, character, and diagnostic prospects. Mol Cell Proteomics 2002;1:845-
                   67.
               10.  Hortin GL, Sviridov D, Anderson NL. High-Abundance Polypeptides of the Human Plasma Proteome Comprising the Top 4 Logs of
                   Polypeptide Abundance. Clin Chem 2008;54:1608-16.
               11.  Lobb RJ, Becker M, Wen Wen S, Wong CSF, Wiegmans AP, et al. Optimized exosome isolation protocol for cell culture supernatant and
                   human plasma. J Extracell Vesicles 2015;4:27031.
               12.  Ludwig AK, De Miroschedji K, Doeppner TR, Börger V, Ruesing J, et al. Precipitation with polyethylene glycol followed by washing and
                   pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales. J Extracell Vesicles
                   2018;7:1528109.
               13.  Chen C, Skog J, Hsu CH, Lessard RT, Balaj L, et al. Microfluidic isolation and transcriptome analysis of serum microvesicles. Lab Chip
                   2010;10:505-11.
   100   101   102   103   104   105   106   107   108   109   110