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Page 4 of 8 Ding et al. Hepatoma Res 2018;4:12 I http://dx.doi.org/10.20517/2394-5079.2018.07

NF-κB relative quantification

1500
c

ΔФ (gray value) 1000 b

500 a

0
A B C D E F
Groups
Figure 1. Detection of non-radioactive NF-κB by EMSA in six groups. Group A: non-transfected, non-treated cells; group B: transient
β2GPI-and HBsAg-transfection without LPS treatment; group C: non-transfected cells treated with 100 ng/mL LPS; group D: transient
β2GPI- and HBsAg-transfection and treated with 100 ng/mL LPS; group E: transient β2GPI-transfection and treated with 100 ng/mL LPS;
group F: transient HBsAg-transfection and treated with 100 ng/mL LPS. β2GPI: beta2-glycoprotein I; HBsAg: hepatitis B surface antigen;
LPS: lipopolysaccharide

A B C D E F

NF-κB

Free probe

Figure 2. NF-κB relative quatification in six groups. Group A: non-transfected, non-treated cells; group B: transient β2GPI-and HBsAg-
transfection without LPS treatment; group C: non-transfected cells treated with 100 ng/mL LPS; group D: transient β2GPI- and HBsAg-
transfection and treated with 100 ng/mL LPS; group E: transient β2GPI-transfection and treated with 100 ng/mL LPS; group F: transient
HBsAg-transfection and treated with 100 ng/mL LPS. Data presented as means ± SD; a: groups B, C, D, E, and F compared with group A,
P < 0.05. b: group B compared with groups A, C, E, and F, P < 0.05, c: group D compared with other five groups, P < 0.05. β2GPI: beta2-
glycoprotein I; HBsAg: hepatitis B surface antigen; LPS: lipopolysaccharide