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Page 4 of 8                                                    Ding et al. Hepatoma Res 2018;4:12  I  http://dx.doi.org/10.20517/2394-5079.2018.07


                                                     NF-κB relative quantification


                                  1500
                                                                       c



                                ΔФ (gray value)  1000  b







                                   500     a






                                     0
                                           A                  B                  C                  D                  E                  F
                                                                     Groups
               Figure 1. Detection of non-radioactive NF-κB by EMSA in six groups. Group A: non-transfected, non-treated cells; group B: transient
               β2GPI-and HBsAg-transfection without LPS treatment; group C: non-transfected cells treated with 100 ng/mL LPS; group D: transient
               β2GPI- and HBsAg-transfection and treated with 100 ng/mL LPS; group E: transient β2GPI-transfection and treated with 100 ng/mL LPS;
               group F: transient HBsAg-transfection and treated with 100 ng/mL LPS. β2GPI: beta2-glycoprotein I; HBsAg: hepatitis B surface antigen;
               LPS: lipopolysaccharide


                                          A            B            C             D            E             F


                                                                                         NF-κB





















                                                                                        Free probe




               Figure 2. NF-κB relative quatification in six groups. Group A: non-transfected, non-treated cells; group B: transient β2GPI-and HBsAg-
               transfection without LPS treatment; group C: non-transfected cells treated with 100 ng/mL LPS; group D: transient β2GPI- and HBsAg-
               transfection and treated with 100 ng/mL LPS; group E: transient β2GPI-transfection and treated with 100 ng/mL LPS; group F: transient
               HBsAg-transfection and treated with 100 ng/mL LPS. Data presented as means ± SD; a: groups B, C, D, E, and F compared with group A,
               P < 0.05. b: group B compared with groups A, C, E, and F, P < 0.05, c: group D compared with other five groups, P < 0.05. β2GPI: beta2-
               glycoprotein I; HBsAg: hepatitis B surface antigen; LPS: lipopolysaccharide
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