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Lai et al. Microbiome Res Rep 2024;3:21 https://dx.doi.org/10.20517/mrr.2023.76 Page 9 of 15

Figure 2. Differential metabolites calculated by OPLS-DA between (A) MF-A and MLS-A and (B) MF-A and MF-E. The selection criteria
of the compounds were those whose VIP value was greater than 2 (A) or 2.5 (B). The principle of sample selection was based on the
***
cluster results in Figure 1B, which were classified into different clusters. Asterisk refers to statistical significance, P < 0.001. MF:
Manjing leaves; MLS: mustard leaf stem; VIP: variable important in projection.

(44.76%~59.77%), while it was relatively less common in MF-B (2.23%) and MF-C (1.00%).
Lactiplantibacillus belongs to Lactobacillus which is a typical genus of most fermented Suancai and is
associated with metabolites of products [2,3,22] . The discrepancies in Lactiplantibacillus abundance alter the