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O’Connell et al. Microbiome Res Rep 2023;2:21 https://dx.doi.org/10.20517/mrr.2023.17 Page 5 of 21

Figure 1. Flow chart illustrating the two classification systems. A summary of the identification of novel genera within the workflow
for identifying novel taxonomy is presented in red and based on the proposed roadmap to genome-based phage taxonomy as
[12]
outlined by Turner et al. (2021) . The workflow for the recognition of novel subclusters within the Actinobacteriophage
Database classification system is presented in blue. In the centre is the proposed overlap between the two systems (purple),
which proposes that a single genus can be assigned to a single subcluster.

Examination of amino acid-based phylogeny using VICTOR to illustrate the monophyletic nature of
the proposed genera and subclusters
Phylogenetic dendrograms based on the amino acid content of the genomes belonging to the proposed
genera were created using VICTOR to provide additional evidence for their creation. VICTOR compares
genome or proteome sequences to generate dendrograms extrapolated from the genome-BLAST distance
phylogeny method with branch support . The most highly supported trees (as calculated by the VICTOR
[23]
server) were closely examined to determine whether the proposed genera were represented on a single
branch, which is the expected presentation based on the roadmap to genome-based taxonomy proposed by
Turner et al. .
[12]