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Gotoh et al. Microbiome Res Rep 2023;2:20 https://dx.doi.org/10.20517/mrr.2023.14 Page 7 of 17
Figure 1. The overall structure of BiBga42A. WT-GOL structure (rainbow color) with bound glycerol (magenta) is shown. LNT in E318S-
LNT structure (yellow) is superimposed [Figure 2]. (A) Monomer structure; (B) Trimer structure related by a crystallographic 3-fold
axis. Trp-200 and Phe-221, which form the substrate binding site of a neighboring subunit, are indicated by arrows. BiBga42A: a
glycoside hydrolase family 42 β-galactosidase; LNT: lacto-N-tetraose; WT-GOL: WT enzyme complexed with glycerol.
unit forms a trimer with crystallographic symmetry-related molecules. The electron density map of LNT
was clearly visible at the active site [Supplementary Figure 1C]. We mainly describe chain B because the
electron density of LNT was more clearly observed. The pyranose sugar ring of the Gal moiety in subsite -1
