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Page 8 of 15 Sasaki et al. Microbiome Res Rep 2023;2:12 https://dx.doi.org/10.20517/mrr.2023.08
indicates characterized enzymes.
Figure 2. AAfase reaction with polysaccharides. TLC (A) and HPAEC-PAD (B) analyses of AAfase reaction with 1% gum arabic AGP
(lane 1), larch AGP (lane 2), wheat AGP (lane 3), and sugar beet arabinan (lane 4). The polysaccharides were incubated in the absence
(-) or presence (+) of AAfase. HPAEC-PAD analysis of the reaction mixture of wheat AGP and sugar beet arabinan is not shown. (C)
[8]
The HPAEC-PAD data for GAfase reaction with gum arabic AGP had been published previously . AA indicates β-L-Arap-(1→3)-L-Ara.
Figure 3. HPAEC-PAD analysis of AAfase reaction with oligosaccharides derived from gum arabic. AGP. HPAEC-PAD analysis of the
reaction with gum arabic-related oligosaccharides. S3-GA (A), S3-AA (B), and S5-GA (C) were incubated with GAfase or AAfase at
37 °C for 24 h.

