Page 56 - Read Online
P. 56

Page 8 of 15                 Sasaki et al. Microbiome Res Rep 2023;2:12  https://dx.doi.org/10.20517/mrr.2023.08


                indicates characterized enzymes.




























                Figure 2. AAfase reaction with polysaccharides. TLC (A) and HPAEC-PAD (B) analyses of AAfase reaction with 1% gum arabic AGP
                (lane 1), larch AGP (lane 2), wheat AGP (lane 3), and sugar beet arabinan (lane 4). The polysaccharides were incubated in the absence
                (-) or presence (+) of AAfase. HPAEC-PAD analysis of the reaction mixture of wheat AGP and sugar beet arabinan is not shown. (C)
                                                                               [8]
                The HPAEC-PAD data for GAfase reaction with gum arabic AGP had been published previously . AA indicates β-L-Arap-(1→3)-L-Ara.




































                Figure 3. HPAEC-PAD analysis of AAfase reaction with oligosaccharides derived from gum arabic. AGP. HPAEC-PAD analysis of the
                reaction with gum arabic-related oligosaccharides. S3-GA (A), S3-AA (B), and S5-GA (C) were incubated with GAfase or AAfase at
                37 °C for 24 h.
   51   52   53   54   55   56   57   58   59   60   61