Page 129 - Read Online

Page 12 of 23 Gutierrez et al. Microbiome Res Rep 2023;2:36 https://dx.doi.org/10.20517/mrr.2023.37

Table 4. Literature review of strain-specific effects of Bifidobacterium species on mucus modulation in the context of inflammation or
infectious disease
Intestinal
Bifidobacterium species Finding Experimental model Ref.
site
B. bifidum FL-276.1 Increased MUC2, improved mucus, reduce Colon DSS-colitis [166]
colitis
B. bifidum FL-228.1 Increased MUC2, improved mucus, reduce Colon DSS-colitis [166]
colitis
B. bifidum BGN4 Improved mucus, reduce colitis Colon DSS-colitis [168]
B. longum subsp. longum YS108R Increased MUC2, improved mucus, reduce Colon DSS-colitis [169]
colitis
B. longum Bif10 Increased MUC2, improved mucus, reduce Colon DSS-colitis [171]
colitis
B. breve Bif11 Increased MUC2, improved mucus, reduce Colon DSS-colitis [171]
colitis
B. breve CBT BR3 Improved mucus, reduce colitis Colon DSS-colitis [170]
B. animalis subsp. lactis A6 Improved mucus, reduce colitis Colon DSS-colitis [167]
B. infantis GMCC0460.1 Improved mucus, reduce colitis Colon DSS-colitis [172]
B. infantis 2017012 Improved mucus, reduce colitis Colon DSS-colitis [176]
B. infantis unclassified strain Improved mucus, reduce colitis Colon DSS-colitis [176]
B. breve H4-2 Improved mucus, reduce colitis Colon DSS-colitis [174]
B. breve H9-3 Improved mucus, reduce colitis Colon DSS-colitis [174]
B. lactis BL-99 Improved mucus, reduce colitis Colon Zebrafish colitis model [177]
B. dentium ATCC 27678 Increased MUC2, improved mucus, reduce Colon TNBS-colitis [96]
colitis
B. infantis unclassified strain Improved mucus, reduce colitis Colon TNBS-colitis [178]
B. longum Bar 33 Improved mucus, reduce colitis Colon TNBS-colitis [176]
B. animalis subsp. lactis CNCM- Improved mucus, reduce colitis Colon DNBS-colitis [179]
I2494
B. bifidum E3 Increased MUC2, improved mucus Small intestine LPS-induced injury [180]
B. infantis E4 Increased MUC2, improved mucus Small intestine LPS-induced injury [180]
B. lactis BB12 Increased MUC2, improved mucus Small intestine LPS-induced injury [180]
B. bifidum OLB637 Increased mucin expression Small intestine Rat model of NEC [181]
B. bifidum G9-1 Increased MUC2, improved mucus Small intestine Rotavirus mouse model [185]
B. infantis PCM Improved mucus Small intestine Cronobacter sakazakii mouse [186]
model

Colitis-inducing compounds are known to activate ER stress [131-134] , and ER stress has been linked to
intestinal inflammation in multiple animal models [135-139] . Goblet cells are particularly sensitive to ER stress
since producing and folding MUC2 is a complex process [140,141] . It has been speculated that modulation of
goblet cell ER stress by Bifidobacterium species may represent a key pathway by which bifidobacteria
promote intestinal health. In mucus-producing Caco-2 cells, the application of live B. breve YIT 12272 and
B. adolescentis YIT 4011T alleviated tunicamycin-induced ER stress . In another study using mucus-
[142]
producing T84 cells, it was shown that B. dentium ATCC 27678-secreted metabolites could also suppress
[96]
tunicamycin- or thapsigarin-induced ER stress . Analysis of the B. dentium metabolites revealed that this
strain generated substantial levels of γ-glutamylcysteine, a compound that can be converted into the
powerful antioxidant glutathione and suppress oxidative and ER stress [131,133,143-147] . B. dentium metabolites
harboring γ-glutamylcysteine and application of commmerically available γ-glutamylcysteine both elevated
glutathione, suppressed inflammatory NF-κB activation, reduced IL-8 secretion, and attenuated the
induction of the unfolded protein response (UPR) genes GRP78, CHOP, and sXBP1 in T84 cells and TNBS-
treated mice . These data suggest that Bifidobacterium species can reduce goblet cell ER stress.
[96]